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Ca2+ imaging analysis of neural circuitry that supports olfactory memory formation in Drosophila

支持果蝇嗅觉记忆形成的神经回路的 Ca2 成像分析

基本信息

批准号：
24770066
负责人：
HIROI Makoto
金额：
$ 2万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (B)
财政年份：
2012
资助国家：
日本
起止时间：
2012-04-01 至 2014-03-31
项目状态：
已结题

项目摘要

To identify neural circuitry that support the memory formation in Drosophila, I analyzed Ca imaging from the brain during olfactory conditioning. I first established a functional imaging system to monitor neuronal activities in the brain of awake, behaving animals responding to sensory stimuli. The in vivo preparation to be established here should provide a consistent way of analyzing gene function during olfactory learning and memory. The functional imaging allows us to analyze temporal and spatial dynamics of many neurons simultaneously. This is an important feature because populations of the ~2500 intrinsic mushroom body neurons will be largely heterogeneous in function and fate after training. In addition, I adopted principal component analysis on the data that provides the opportunity for more comprehensive and detailed analyses of cell physiology that will expand our understanding of memory storage.

为了确定支持果蝇记忆形成的神经回路，我分析了嗅觉条件反射过程中大脑的钙成像。我首先建立了一个功能成像系统，以监测清醒的动物大脑中对感官刺激做出反应的神经元活动。在这里建立的体内制备应该提供一个一致的方式分析基因功能在嗅觉学习和记忆。功能成像使我们能够同时分析多个神经元的时间和空间动力学。这是一个重要的特征，因为约2500个内在蘑菇体神经元的群体在训练后在功能和命运上将是很大程度上异质的。此外，我采用了主成分分析的数据，提供了更全面和详细的细胞生理学分析的机会，这将扩大我们对记忆存储的理解。