Molecular mechanism of petal movement

花瓣运动的分子机制

• 批准号: 24780029
• 负责人: NAKATSUKA Takashi
• 金额: $ 2.91万
• 依托单位: Shizuoka University (2013)Iwate Biotechnology Research Center (2012)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24780029/
• 关键词: 開閉運動; アクアポリン; リンドウ; 花き品質; 花冠開閉

The closure of petals is one of the problems in the potted gentian. This study was attempted to reveal the mechanism of petal movement. When the incubation condition was changed from 25 to 15 degrees, the petals of potted gentian closed rapidly. On the other hand, when it was changed from 15 to 25 degrees, the petals opened slowly throughout 2 hours.We identified 15 aquaporin genes, encoding water pomp protein, from gentian petal cDNA library. The expressions of some aquaporin genes categorized into PIP2 subfamily were detected strongly in floral organs of gentian plants. However, the significant difference of PIP2 transcript and protein accumulations between opening and closure petals could not be observed.

花瓣闭合是盆栽龙胆存在的问题之一。本研究试图揭示花瓣运动的机理。当培养条件由25 ℃变为15 ℃时，盆栽龙胆花瓣迅速闭合。从龙胆花瓣cDNA文库中筛选到15个编码水囊蛋白的水通道蛋白基因。在龙胆属植物花器官中检测到一些属于PIP2亚家族的水通道蛋白基因的强表达。而PIP2的转录和蛋白质积累在花瓣开放和关闭时没有显著差异。

项目成果

リンドウcDNAライブラリーの構築

龙胆cDNA文库的构建

• 发表时间: 2013
• 作者: 中塚貴司;山田恵理;今村智弘;高橋秀行;斎藤美沙;藤田晃平;西原昌宏
• 通讯作者: 西原昌宏

静岡大学農学部 花き園芸学研究室

静冈大学农学部花卉园艺研究室

Suppression of anthocyanin pigmentation by single MYB transcription factors from gentian

龙胆单 MYB 转录因子抑制花青素色素沉着

• 发表时间: 2013
• 期刊: Plant Cell Reports
• 影响因子: 6.2
• 作者: Nakatsuka T;Yamada E;Saito M;Fujita K;Nishihara M.
• 通讯作者: Nishihara M.