Study of Mg distribution and Mg transporter using radioisotope as a tracer

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We observed magnesium (Mg) movement in rice plant with a short-life nuclide, magnesium 28 (28Mg: half-life is 20.9 hour) as a radioactive tracer. 28Mg transport drastically decreased in the 2nd newest leaf on day 6 of Mg-free nutrient culture. As a result from the further analysis of the leaf, it was indicated that the defect in transpiration flow was found earlier than the other symptoms of Mg deficiency, such as photosynthesis reduction and necrosis. When we analyzed the Mg uptake manner in root, we found that Mg uptake activity was decreased by low pH. In addition, 28Mg experiment showed the different magnesium uptake and transport activity along the rice root axis.We also performed a complementation assay of rice MRS2 family in the yeast CM66 strain, which lacks Mg uptake ability. As a result, OsMRS2-6 was shown to have an ability of Mg transport.