Spatiotemporal analysis of lipids in avian yolk

点击翻译按钮获取中文摘要

In the previous study, we visualized phosphatidylcholine (PC) molecular species by MALDI imaging mass spectrometry (IMS). They formed characteristic ring structures in the quail yellow yolk that were categorized by the type of fatty acid contained in the PC molecular species. The ring structures formed by the PC molecular species were distinct from the known ring structure formed by dark and light bands.In this study, we attempted to visualize other phospholipids by MALDI-IMS. Phosphatidylethanolamine (PE), phosphatidylinositol (PI), lysophosphatidylcholine (LPC), lysophosphatidylethanolamine (LPE), and sphingomyelin (SM) molecular species are visualized. PE and PI molecular species also formed characteristic ring structures in the quail yellow yolk. Furthermore, most of SM molecular species are distributed in the germinal vesicle and latebra.