Regulation of species--dependent calcium oscillations during fertilization in mammals

哺乳动物受精过程中物种依赖性钙振荡的调节

• 批准号: 24780272
• 负责人: ITO JUNYA
• 金额: $ 2.91万
• 依托单位: Azabu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24780272/
• 关键词: 受精

We succeeded in molecular cloning of horse PLCz. We also showed that horse PLCz has higher activity compared to those in other mammalian species studied to date. As for the regulation of IP3R in pig oocytes, inhibition of polo-like kinase 1(Plk1) dramatically decreased the expression level of IP3R, suggests that Plk1 is involved in the regulation of IP3R expression at least in pig oocytes.

我们成功地分子克隆了马PLCz。我们还表明，与迄今为止研究的其他哺乳动物物种相比，马 PLCz 具有更高的活性。至于猪卵母细胞中IP3R的调节，抑制polo样激酶1（Plk1）可显着降低IP3R的表达水平，表明Plk1至少参与了猪卵母细胞中IP3R表达的调节。

项目成果

The Ca^<2+> signal at fertilization in mammals: PLC, sterility, and species-specific differences in activity. Sperm Cell Research in the 21st Century: Historical Discoveries to New Horizons Chapter 10:

哺乳动物受精时的Ca ^ 2 信号：PLC、不育性和活性的物种特异性差异。

• 发表时间: 2012
• 作者: Junya Ito;Yuki Yamashita;Koji Furukawa;Ayaka Nakamura;Kana Sato;Akiko Takizawa;Hoi Chang Lee;Rafael A. Fissore;Naomi Kashiwazaki;Junya Ito and Rafael A. Fissore
• 通讯作者: Junya Ito and Rafael A. Fissore

Improvement of in vitro and in vivo development of vitrified mammalian oocytes and embryos

玻璃化哺乳动物卵母细胞和胚胎的体外和体内发育的改善

• 发表时间: 2012
• 作者: Junya Ito;Emi Yuhara;Ayaka Nakamura;Naomi Kashiwazaki;Junya Ito
• 通讯作者: Junya Ito

Characteristics of transgenic rats expressing horse PLCz

表达马PLCz的转基因大鼠的特征

• 发表时间: 2013
• 作者: Junya Ito;Yuki Yamashita;Koji Furukawa;Ayaka Nakamura;Kana Sato;Akiko Takizawa;Hoi Chang Lee;Rafael A. Fissore;Naomi Kashiwazaki
• 通讯作者: Naomi Kashiwazaki

Sperm Cell Research in the 21st Century: Historical Discoveries to New Horizons

21世纪的精子细胞研究：历史发现到新视野

• 发表时间: 2012
• 作者: Junya Ito;Yuki Yamashita;Koji Furukawa;Ayaka Nakamura;Kana Sato;Akiko Takizawa;Hoi Chang Lee;Rafael A. Fissore;Naomi Kashiwazaki;Junya Ito and Rafael A. Fissore;Junya Ito and Rafel A. Fissore
• 通讯作者: Junya Ito and Rafel A. Fissore

The effect of M-phase stage-dependent kinase inhibitors on inositol 1,4,5-trisphosphate receptor 1 (IP3 R1) expression and localization in pig oocytes.

• DOI: 10.1111/asj.12258
• 发表时间: 2015-02
• 期刊: Animal science journal = Nihon chikusan Gakkaiho
• 作者: A. Sathanawongs;Katsuyoshi Fujiwara;Tsubasa Kato;M. Hirose;M. Kamoshita;R. Wojcikiewicz;J. Parys;J. Ito;N. Kashiwazaki