Inhibition of intracellular transport involved in fluoride-induced hepatotoxicity.

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Fluoride hepatotoxicity reveals lipid retention in hepatocyte. We assumed that a disruption of intra-cellular lipid vesicle transport retained the lipid in endoplasmic reticulum. We examined the role of lipid metabolism relating proteins CD36, ApoB, CIDE-B, Vamp-7, FABP-1 in intra-cellular vesicle traffic systems. CIDE-B was recruited from cytosol to endoplasmic reticulum membrane with the formation of protein transport vesicle. On the other hand, inhibition of protein transport vesicle formation blocked CIDE-B recruitment from cytosol to endoplasmic reticulum membrane. These results indicate lipid metabolism relating protein CIDE-B recruited from cytosol to endoplasmic reticulum membrane like a coat protein of protein transport vesicles.