Analysis of atypical BSE prions propagation in bovine prion protein overexpression mice

牛朊病毒蛋白过表达小鼠中非典型疯牛病朊病毒繁殖分析

• 批准号: 24780302
• 负责人: MIYAZAWA Kohtaro
• 金额: $ 2.33万
• 依托单位: National Agriculture and Food Research Organization
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24780302/
• 关键词: 非定型BSE; 定型BSE; バイオアッセイ; PrPScの経時変化; 感染価の経時変化; 感染価の増幅; プリオン株

In time course study of L-BSE and H-BSE inoculation using bovine PrP overexpression mice, PrPSc was first detectable after 60-70 days post inoculation. L-BSE affected mice with a short incubation time accumulated approximately 18-fold higher PrPSc than H-BSE affected mice with a long incubation time. However, they had similar infectious titer at terminal stage of illness. The kinetics study using wild-type mice inoculated with mouse-adapted BSE brain homogenate (mBSE-BH) showed the discrepancy between the infectivity and PrPSc accumulation. Approximately 99% of PrPSc in crude mBSE-BH was precipitated by 20K xg centrifugation. Nevertheless, approximately 63% of infectivity was recovered in the supernatant (20K-supe). Interestingly, the incubation time of mice inoculated with 20K-supe was significantly longer than that of mice inoculated with crude mBSE-BH.These results indicate that different types of PrPSc may play important roles in mBSE infectivity and disease state, respectively.

在使用牛PrP过表达小鼠的L-BSE和H-BSE接种的时间过程研究中，在接种后60-70天后首次检测到PrPSc。孵育时间短的L-BSE感染小鼠的PrPSc蓄积量约为孵育时间长的H-BSE感染小鼠的18倍。但在疾病末期，它们的感染性滴度相似。使用野生型小鼠接种小鼠适应性BSE脑匀浆（mBSE-BH）的动力学研究表明，感染性和PrPSc积累之间的差异。通过20 K xg离心沉淀粗mBSE-BH中约99%的PrPSc。然而，在上清液（20 K-supe）中回收了约63%的感染性。有趣的是，20 K-supe接种小鼠的潜伏期明显长于mBSE-BH粗品接种小鼠的潜伏期。这些结果表明，不同类型的PrPSc可能分别在mBSE的感染性和疾病状态中起重要作用。

项目成果

Infectivity, neurotoxicity and PrP^<Sc> accumulation in BSE-affected mice

受 BSE 影响的小鼠的感染性、神经毒性和 PrP^<Sc> 积累

• 发表时间: 2014
• 作者: Kohtaro Miyazawa;Hiroyuki Okada;Kentaro Masujin and Takashi Yokoyama
• 通讯作者: Kentaro Masujin and Takashi Yokoyama

Infectivity, neurotoxicity and PrPSc accumulation in BSE-affected mice

受 BSE 影响的小鼠的感染性、神经毒性和 PrPSc 积累

• 发表时间: 2014
• 作者: Kohtrao Miyazawa;Hiroyuki Okada;Kentaro Masujin;Takashi Yokoyama
• 通讯作者: Takashi Yokoyama