Membrane localization of LRRK2, a causative gene for familial Parkinson's

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It has been reported that LRRK2 exists on membrane of organelles or vesicles in cytoplasm by electron microscopic analysis. However, the membrane localization of LRRK2 has not been precisely characterized. In this study, we found that asmall fraction of LRRK2 was eluted in the detergent-soluble membrane fraction. We also found that LRRK2 translocates to the LAMP1-positive structure upon treatment of cells with a LRRK2 inhibitor. In addition, we succeeded in forcing LRRK2 to translocate to organelles by using a chemical-induced localization system.