Membrane localization of LRRK2, a causative gene for familial Parkinson's

电镜分析表明，LRRK2存在于细胞器膜或细胞质囊泡上。然而，LRRK2的膜定位尚未被精确表征。在本研究中，我们发现在洗涤剂溶膜组分中有一小部分LRRK2被洗脱。我们还发现，在使用LRRK2抑制剂处理细胞后，LRRK2易位到lamp1阳性结构。此外，我们成功地利用化学诱导的定位系统迫使LRRK2转移到细胞器。

It has been reported that LRRK2 exists on membrane of organelles or vesicles in cytoplasm by electron microscopic analysis. However, the membrane localization of LRRK2 has not been precisely characterized. In this study, we found that asmall fraction of LRRK2 was eluted in the detergent-soluble membrane fraction. We also found that LRRK2 translocates to the LAMP1-positive structure upon treatment of cells with a LRRK2 inhibitor. In addition, we succeeded in forcing LRRK2 to translocate to organelles by using a chemical-induced localization system.