Identification of the molecule which controls lymphangiogenesis and research of the medical treatment method for lymph node metastasis and lymphedema

控制淋巴管生成的分子的鉴定及淋巴结转移和淋巴水肿的治疗方法的研究

• 批准号: 24790312
• 负责人: SENDA kazutaka
• 金额: $ 2.91万
• 依托单位: University of Toyama
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24790312/
• 关键词: リンパ管新生; 肺癌; 分子腫瘍学; 腫瘍生物学

For evaluation of lymphangiogenesis, lymph node metastases, pulmonary metastasis, we established the luciferase stability expression human lung cancer cell line (A549). We examined the intracellular signals in lung cancer cells. We identified the compound which had an influence on the activity of HIF1a which was important to angiogenesis and lymphangiogenesis. We examined the effects of some compounds on lymphangiogenesis inhibition and promotion. Also, A549 luciferase stability expression cells were transfused into mice and we examined the effects of these compounds on tumor growth, lymphangiogenesis, lymph node metastases, pulmonary metastasis.

为了评价淋巴管生成、淋巴结转移、肺转移，我们建立了稳定表达荧光素酶的人肺癌细胞系(A549)。我们检测了肺癌细胞内的信号。我们鉴定了对HIF1a活性有影响的化合物，HIF1a对血管生成和淋巴管生成具有重要作用。我们检测了一些化合物对淋巴管生成的抑制和促进作用。此外，将A549荧光素酶稳定表达细胞导入小鼠体内，我们检测了这些化合物对肿瘤生长、淋巴管生成、淋巴转移、肺转移的影响。

项目成果

Calpain 1 and -2 play opposite roles in cord formation of lymphatic endothelial cells via eNOS regulation

• DOI: 10.1007/s13577-012-0042-7
• 发表时间: 2012-02
• 期刊: Human Cell
• 影响因子: 4.3
• 作者: Orawin Prangsaengtong;K. Senda;Y. Doki;J. Park;M. Jo;H. Sakurai;N. Shibahara;I. Saiki;K. Koizumi-K