Search for the marker of chandelier cells in cerebral cortex

吊灯细胞是支配锥体神经元轴突起始段的最独特的GABA能神经元。尽管有特殊的形态和功能，但没有发现枝形吊灯神经元标记物。最近，有报道称，在胚胎第15天来自vMGE的移植中，枝形吊灯细胞得到了丰富(Inan等人，2012年)。此外，在Nkx2-1Creer小鼠中还标记了一些皮质枝形吊灯细胞(Taniguchi等人，2013年)。为了寻找吊灯神经元标记物，我们将BAC-PV-Cre转基因小鼠来源的E15.5vMGE细胞移植到P0小鼠大脑皮质。尽管如此，在移植的成年小鼠皮质中没有发现枝形吊灯细胞。接下来，我们将他莫昔芬注射到E17.5 Nkx2-1Creer；R26-YFP小鼠体内，用于标记吊灯细胞。结果显示，在P0小鼠的VZ和SVZ内可见GFP阳性祖细胞。未来，我们计划挑选GFP阳性前体细胞，并尝试单细胞微阵列分析。

The chandelier cells are the most distinct GABAergic neurons that innervate the axon initial segment of pyramidal neurons. In spite of particular morphology and function, there is no chandelier neuron markers were found. Recently, it has been reported that the chandelier cell is enriched in transplants from the vMGE at embryonic day 15 (Inan et al., 2012). In addition, a number of cortical chandelier cells were labeled in Nkx2-1CreER mouse (Taniguchi et al., 2013). To search chandelier neuron marker, we transplanted the E15.5 vMGE cells that derived from BAC- PV- Cre transgenic mouse to P0 mouse cortex. Nonetheless, there are no chandelier cells were found in the transplanted adult mouse cortex. Next, we injected tamoxifen to E17.5 Nkx2-1CreER; R26-YFP mouse for labeling chandelier cells. As the results, GFP positive progenitor cells were found in VZ and SVZ in the P0 mouse. In future, we plan to pick up GFP positive progenitor and try single-cell microarray analysis.