Establishment of safe and efficient differentiation system using automatic removal vector

当我们从多能干细胞诱导分化细胞时，必须考虑到残留的未分化细胞形成肿瘤的风险。为了减少这种风险，我们试图利用我们的SeVdp载体系统建立一个系统来消除分化组织中的未分化细胞。将含有神经元特异性miRNA靶序列的SeVdp载体感染小鼠iPS细胞，诱导其分化为神经元。结果，当载体在未分化的细胞中持续感染时，它会自动从神经元中移除。但当我们尝试用负选择基因从载体中筛选分化细胞时，不仅未分化的细胞被杀死，而且分化的细胞也被杀死。我们计划探索其他分化和负选择的方法，选择性地消除未分化的细胞。

When we induce differentiated cells from pluripotent stem cells, the risks of tumor formation by remained undifferentiated cells must be concerned. To reduce such a risk, we tried to establish a system for elimination of undifferentiated cells in the differentiated tissue using our SeVdp vector system. The SeVdp vector which has the target sequence of neuron-specific miRNA was infected to mouse iPS cells, and then they were differentiated to neuron. As a result, the vector were automatically removed from neuron while it was persistently infected in undifferentiated cells. But when we tried to select differentiated cells using negative selection gene from the vector, not only undifferentiated cells but also differentiated cells were killed by the treatment. We plan to investigate other method of differentiation and negative selection to selectively eliminate the undifferentiated cells.