Early detection of disease using surface enhance Raman scattering (SERS)-dynamic gold nanoparticle to detect inflammatory response in cells

使用表面增强拉曼散射 (SERS) 动态金纳米颗粒检测细胞中的炎症反应，早期检测疾病

• 批准号: 24700481
• 负责人: PISSUWAN Dakrong
• 金额: $ 2.91万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24700481/
• 关键词: 金ナノ粒子; SERS; 炎症反応; ICAM-1; サイトカイン

The investigation of the use of surface enhanced Raman scattering (SERS) gold nanoparticles for rapid monitoring of inflammatory responses at the early stage of diseases. Gold nanorods (GNRs) combined with Raman probes (4-mercaptobenzoic acid) are conjugated with intercellular adhesion molecule-1 antibody (anti-ICAM-1). The prepared particles could be used as a SERS probe to detect the expression of ICAM-1 in macrophages treated lipopolysaccharide for 1 h which is faster than using ELISA or fluorescent labeling techniques.

利用表面增强拉曼散射（Sers）金纳米粒子快速监测疾病早期炎症反应的研究。将金纳米棒（GNRs）与拉曼探针（4-巯基苯甲酸）结合，与细胞间粘附分子-1抗体（抗ICAM-1）缀合。制备的颗粒可作为Sers探针检测脂多糖处理1h后巨噬细胞ICAM-1的表达，比ELISA或荧光标记技术更快。

项目成果

Gold nanoparticles for the development of transdermal delivery system, Functional Nanoparticles for Bioanalysis, Nanomedicine, and Bioelectronic Devices Volume 2

用于开发透皮给药系统的金纳米颗粒、用于生物分析、纳米医学和生物电子设备的功能性纳米颗粒第 2 卷

• 发表时间: 2012
• 作者: Pissuwan;D.;Niidome;T.
• 通讯作者: T.

The uptake and intracellular signal monitoring in macrophage cells of different surface modified gold nanorods using surface enhanced Raman scattering spectroscopy

利用表面增强拉曼散射光谱监测巨噬细胞对不同表面修饰金纳米棒的摄取和细胞内信号

• 发表时间: 2013
• 作者: Pissuwan;D.;Hobro;A.;Pavillon;N.;Smith;N.
• 通讯作者: N.

Gold nanorods : Tool to detect early stage inflammation and their surface effects on inflammatory response in macrophage cells

金纳米棒：检测早期炎症及其表面对巨噬细胞炎症反应影响的工具

• 发表时间: 2013
• 作者: Pissuwan;D.;Smith;N.
• 通讯作者: N.

Effect of different surface modifications of gold nanorods on inflammatory cytokine induction in macrophage cells

金纳米棒不同表面修饰对巨噬细胞炎症细胞因子诱导的影响

• 发表时间: 2012
• 作者: Pissuwan;D.;Smith;N.
• 通讯作者: N.

The uptake and intracellular signal monitoring in macrophage cells of different surface modified gold nanorods using surface-enhanced Raman scattering spectroscopy

使用表面增强拉曼散射光谱监测巨噬细胞对不同表面修饰金纳米棒的摄取和细胞内信号

• 发表时间: 2013
• 作者: Pissuwan;D.;Hobro;A.;Pavillon;N.;Smith;N.
• 通讯作者: N.