Study of anti-aging mechanism of the exercise from protein acetylation

从蛋白质乙酰化角度探讨运动抗衰老的机制。老年大鼠每天跑台运动60 min，每周4次，共8周。分析肝蛋白的变化。2D-DIGE（two-dimensional difference gel electrophoresis）显示13个蛋白质点发生了显著变化（10个增加，3个减少）。但这些斑点的变化都在2倍以下，而且每个斑点的强度都很弱。抗乙酰赖氨酸抗体免疫印迹分析显示，随着年龄的增长，肝线粒体蛋白质乙酰化程度降低，而运动大鼠肝线粒体蛋白质乙酰化程度增加。

We studied anti-aging mechanism of exercise via protein acetylation. Aged rats were exercised on treadmill 60 min/day and 4 times per week, for 8 weeks. We analyzed changes of liver protein. 2D-DIGE (two-dimensional difference gel electrophoresis) showed 13 significantly changed protein spots (10 increased and 3 decreased) from a total of 662 spots. However, these spots were below two fold changes, and intensity of each spot was very weak. Protein acetylation of liver mitochondria decreased with age by western blot analysis with anti-acetyl lysine antibody, however, exercised rats increased acetylation of protein.