Study of anti-aging mechanism of the exercise from protein acetylation

我们通过蛋白质乙酰化来研究运动的抗衰老机制。老年大鼠在跑台上运动，60min/d，每周4次，共8周。分析肝脏蛋白质的变化。2D-DGE(双向差示凝胶电泳法)共检测到662个蛋白质点，其中13个蛋白质点有明显变化(10个增加，3个减少)。然而，这些斑点都在两倍变化以下，且每个斑点的强度都很弱。用抗乙酰赖氨酸抗体进行的蛋白质印迹分析表明，随着年龄的增长，肝线粒体的蛋白质乙酰化程度降低，而运动后的大鼠肝线粒体蛋白质乙酰化程度增加。

We studied anti-aging mechanism of exercise via protein acetylation. Aged rats were exercised on treadmill 60 min/day and 4 times per week, for 8 weeks. We analyzed changes of liver protein. 2D-DIGE (two-dimensional difference gel electrophoresis) showed 13 significantly changed protein spots (10 increased and 3 decreased) from a total of 662 spots. However, these spots were below two fold changes, and intensity of each spot was very weak. Protein acetylation of liver mitochondria decreased with age by western blot analysis with anti-acetyl lysine antibody, however, exercised rats increased acetylation of protein.