The effect of food component on anticancer drug-induced alpha-defensin reduction in the intestine.

许多癌症患者正在使用补充剂来减轻化疗的副作用和与治疗相关的症状，如免疫功能受损。一些营养食品已被批准为癌症患者特殊医疗用途的饮食食品。补充剂与抗癌药物之间的相互作用可增强或减弱其药理或毒理作用。因此，我们利用Caco-2细胞株研究了抗癌药物和食物成分之间的相互作用对α-防御素表达的影响。我们检测了Caco-2细胞中HD-5和HD-6的mRNA水平，并评价了FT和食物成分之一的EGCG对这些mRNA水平的影响。FT可降低HD-5和HD-6mRNA的表达水平。FT和过氧化氢均可诱导细胞产生ROS，EGCG可抑制FT诱导的ROS产生。此外，EGCG几乎完全抑制FT诱导的HD-5和HD-6基因表达的降低。

Supplements are being used by many cancer patients to alleviate side effects of chemotherapy and therapy-related symptoms such as immune compromise. Some nutraceuticals have been approved as dietary foods for special medical purposes for cancer patients. The interactions between supplements and anticancer drugs may increase or decrease the pharmacological or toxicological actions. So, we examined the effect of interaction between anticancer drug and food constituent on the expression of alpha-defensins by using a Caco-2 cell line. We detected HD-5 and HD-6 mRNA in Caco-2 cells and evaluated the effects of FT and EGCG, one of food component, on these mRNA levels. HD-5 and HD-6 mRNA levels were decreased by exposure to FT. Production of reactive oxygen species (ROS) was induced by exposure to FT as well as hydrogen peroxide exposure, and EGCG suppressed FT-induced production of ROS. Furthermore, FT-induced decrease in HD-5 and HD-6 mRNA levels was almost completely suppressed by EGCG.