The effect of food component on anticancer drug-induced alpha-defensin reduction in the intestine.

食物成分对抗癌药物引起的肠道α-防御素减少的影响。

• 批准号: 24700818
• 负责人: TAKAHASHI Natsuko
• 金额: $ 2.91万
• 依托单位: Hokkaido Pharmaceutical University School of Pharmacy
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24700818/
• 关键词: 相互作用; 抗がん剤; サプリメント

Supplements are being used by many cancer patients to alleviate side effects of chemotherapy and therapy-related symptoms such as immune compromise. Some nutraceuticals have been approved as dietary foods for special medical purposes for cancer patients. The interactions between supplements and anticancer drugs may increase or decrease the pharmacological or toxicological actions. So, we examined the effect of interaction between anticancer drug and food constituent on the expression of alpha-defensins by using a Caco-2 cell line. We detected HD-5 and HD-6 mRNA in Caco-2 cells and evaluated the effects of FT and EGCG, one of food component, on these mRNA levels. HD-5 and HD-6 mRNA levels were decreased by exposure to FT. Production of reactive oxygen species (ROS) was induced by exposure to FT as well as hydrogen peroxide exposure, and EGCG suppressed FT-induced production of ROS. Furthermore, FT-induced decrease in HD-5 and HD-6 mRNA levels was almost completely suppressed by EGCG.

许多癌症患者正在使用补充剂来减轻化疗的副作用和与治疗相关的症状，如免疫功能低下。一些营养保健品已被批准作为癌症患者特殊医疗用途的膳食食品。补充剂和抗癌药物之间的相互作用可能会增加或减少药理学或毒理学作用。因此，我们通过使用Caco-2细胞系检测抗癌药物和食物成分之间的相互作用对α-防御素表达的影响。我们检测了Caco-2细胞中HD-5和HD-6的mRNA，并评估了FT和食物成分之一的表没食子酸没食子酸酯（表没食子酸酯）对这些mRNA水平的影响。HD-5和HD-6 mRNA水平降低暴露于FT。暴露于FT以及过氧化氢暴露诱导活性氧（ROS）的产生，并且EGCG抑制FT诱导的ROS产生。此外，FT诱导的HD-5和HD-6 mRNA水平的降低几乎完全被EGCG抑制。

项目成果

Immunoprotective effect of epigallocatechin-3-gallate on oral anticancer drug-induced α-defensin reduction in Caco-2 cells.

• DOI: 10.1248/bpb.b13-00700
• 发表时间: 2014-03
• 期刊: Biological & pharmaceutical bulletin
• 影响因子: 2
• 作者: N. Takahashi;Masaki Kobayashi;Jiro Ogura;Hiroaki Yamaguchi;T. Satoh;Kazuhiro Watanabe;K. Iseki

Epigallocatechin gallateがtegafurによる腸管免疫低下 および吸収に与える影響

表没食子儿茶素没食子酸酯对替加氟引起的肠道免疫降低和吸收的影响

• 发表时间: 2014
• 作者: 高橋夏子;澤田真衣;小林正紀;佐藤隆司;渡辺一弘;井関健
• 通讯作者: 井関健

テガフールの腸管吸収機構に関する研究

替加氟肠道吸收机制研究

• 发表时间: 2012
• 作者: 澤田真衣;高橋夏子;小林正紀;佐々木将太郎;井関健.
• 通讯作者: 井関健.

Epigallocatechin gallateがtegafurによる腸管免疫低下および吸収に与える影響

表没食子儿茶素没食子酸酯对替加氟引起的肠道免疫降低和吸收的影响

• 发表时间: 2014
• 作者: 高橋夏子;澤田真衣;小林正紀;佐藤隆司;渡辺一弘;井関健
• 通讯作者: 井関健