Tissue specificity of Carcinoembryonic antigen

癌胚抗原的组织特异性

• 批准号: 24701014
• 负责人: NAKAO Kanako
• 金额: $ 2.16万
• 依托单位: Shizuoka Cancer Center Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24701014/
• 关键词: CEA; 糖鎖修飾; 腫瘍マーカー; プロテオーム

Carcinoembryonic antigen (CEA) is an oncofetal cell surface glycoprotein. Because of its high expression in tumors and secretion to serum, CEA has been widely used as a serum tumor marker. To investigate the tissue specificity of CEA, we examined the expression patterns of CEACAM family members and the N-glycosylation patterns on CEA molecules derived from several cancer cell lines using a proteomic strategy. We identified novel N-glycosylation sites on CEA and further precise investigation revealed that the N-glycosylation patterns were varying among various cancer cell lines. Furthermore, two novel CEA splice variants were identified in gastrointestinal cancer cell lines. Those transcripts were not expressed in normal tissues. CEA protein isoforms derived from the novel splice variants were also expressed in cancer cell lines, and those protein isoforms were secreted to culture medium. We also found that CEACAM4 expression was unique to a medullary thyroid carcinoma cell line.

癌胚抗原(CEA)是一种癌胎儿细胞表面糖蛋白。CEA因其在肿瘤中的高表达和对血清的分泌而被广泛用作血清肿瘤标记物。为了研究CEA的组织特异性，我们用蛋白质组学的方法研究了CEACAM家族成员的表达模式和几种癌细胞系CEA分子上的N-糖基化模式。我们在CEA上发现了新的N-糖基化位点，进一步的精确研究表明，N-糖基化模式在不同的癌细胞系中是不同的。此外，在胃肠道癌细胞系中发现了两个新的CEA剪接变异体。这些转录本在正常组织中不表达。从新的剪接变异体衍生的CEA蛋白亚型也在癌细胞中表达，这些蛋白亚型被分泌到培养液中。我们还发现CEACAM4的表达是甲状腺髓样癌细胞系所特有的。

项目成果

消化器癌由来細胞株における新規CEACAM5アイソフォームタンパク質の同定

胃肠道癌衍生细胞系中新型 CEACAM5 亚型蛋白的鉴定

• 发表时间: 2013
• 作者: 中尾香菜子;畠山慶一;大島啓一;山口建;望月徹
• 通讯作者: 望月徹

Synthesis of Proneurotensin/neuromedin N (proNT/NMN) N-Terminal-Related Peptides to Develop a ProNT/NMN-Specific ELISA System

合成神经降压素原/神经调节素 N (proNT/NMN) N 末端相关肽以开发 ProNT/NMN 特异性 ELISA 系统

• 发表时间: 2014
• 期刊: Peptide Science 2013
• 作者: Sakura N.;Ide T.;Wakabayashi-Nakao K.;Ohshima K.;Hatakeyama K.;Ogura S.;Mochizuki T.;editors
• 通讯作者: editors

CEACAM4の甲状腺髄様癌における特異的発現

CEACAM4在甲状腺髓样癌中的特异性表达

• 发表时间: 2012
• 作者: Matsushita H;Nakamura N;Tanaka Y;Ohgiya D;Tanaka Y;Damdinsuren A;Asai S;Yabe M;Kawada H;Ogawa Y;Ando K;Miyachi H.;中尾香菜子
• 通讯作者: 中尾香菜子

Methods to examine the impact of nonsynonymous SNPs on protein degradation and function of human ABC transporter.

• DOI: 10.1007/978-1-62703-435-7_15
• 发表时间: 2013
• 期刊: Methods in molecular biology
• 作者: T. Ishikawa;Kanako Wakabayashi-Nakao;H. Nakagawa

CEACAM5遺伝子のスプライシングバリアント

CEACAM5基因的剪接变体

• 发表时间: 2013