Speciation, niche differentiation and reproductive isolation in European cereal leaf beetles (Genus Oulema, Coleoptera, Chrysomelidae)

欧洲谷类叶甲虫（Oulema 属、鞘翅目、叶甲科）的物种形成、生态位分化和生殖隔离

• 批准号: 5423529
• 负责人: Professorin Dr. Susanne Dobler
• 金额: --
• 依托单位: Abteilung Molekulare Evolutionsbiologie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2004
• 资助国家: 德国
• 起止时间: 2003-12-31 至 2007-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5423529?language=en
• 关键词: Speciation; niche; differentiation; reproductive; isolation

Phytophagous insects represent not only one of the major radiations on earth but also comprise some of the strongest cases for sympatric speciation to date. Two crucial steps in the formation of new species, niche differentiation and reproductive isolation between emerging species, can especially easily be delimited and tested here. This proposal aims to trace both steps using cereal leaf beetles (Oulema spp.) as a model system. The genus Oulema comprises several grass (Graminaceae) feeding species in Europ e. Based on variation in external and genitalic morphology, it has been claimed that the cereal pest species, O. melanopus, includes a second cryptic species, O. rufocyanea. Through a combination of breeding, rearing and food choice experiments, we plan to establish whether these two sympatric forms actually constitute separate species or rather incipient species that are currently diverging in their feeding habits and reproductive characters. Morphological scoring of presumed diagnostic characters and gene tic analysis of gene flow between the two forms by AFLP (amplified fragment length polymorphisms) will complement the data. To trace the order of speciation events that were or were not associated with host shifts in the European Oulema species, a phylogenetic analysis based on mtDNA sequences will be undertaken.

植食性昆虫不仅代表了地球上的主要辐射之一，而且还包括迄今为止同域物种形成的一些最强案例。新物种形成的两个关键步骤，新物种之间的生态位分化和生殖隔离，可以特别容易地界定和测试。这项建议的目的是跟踪这两个步骤使用谷物叶甲虫（Oulema属）。作为一个模型系统。欧列玛属（Oulema）包括欧洲的几种禾本科（Graminaceae）食草物种。根据外部和生殖器形态上的变异，有人认为禾谷类害虫种O。melanopus包括第二个隐种O.红蓝。通过结合育种，饲养和食物选择实验，我们计划建立这两个同域形式实际上是否构成单独的物种或更确切地说，目前正在分化的食性和生殖特征的初期物种。假定诊断特征的形态学评分和通过AFLP（扩增片段长度多态性）对两种形式之间的基因流动进行基因分析将补充数据。为了追踪物种形成事件的顺序，或不与主机转移在欧洲Oulema物种，系统发育分析的基础上mtDNA序列将进行。