Studies on molecular mechanics of Candida albicans virulence gene regulation by the TEA transcription factor CaTec1p

TEA转录因子CaTec1p调控白色念珠菌毒力基因的分子机制研究

• 批准号: 5426729
• 负责人: Privatdozent Dr. Klaus Schröppel
• 金额: --
• 依托单位: Institut für Medizinische Mikrobiologie und Hygiene
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2004
• 资助国家: 德国
• 起止时间: 2003-12-31 至 2006-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5426729?language=en
• 关键词: Studies; molecular; mechanics; Candida; albicans

The conserved TEA transcription factor CaTec1p regulates morphogenetic development and virulence attributes of the fungal pathogen Candida albicans. Over-expression of CaTEC1 rescues virulence defects of several types of signal transduction mutants and exacerbates virulence of a C. albicans wild-type strain. The proposed research project will investigate the underlying mechanisms of i. induction of specific target promoters by CaTec1p, ii. the structure-function relationship of CaTec1p, and iii. interaction of CaTec1p with other regulatory proteins. The promoters of CaTec1p-dependent genes will be used to identify a CaTec1p-binding DNA motif. This motif will be further analysed during hyphal formation and infection with an in vivo expression reporter system. Different functions will be assigned to the deduced amino acid sequence of the CaTec1p protein by functional complementation of the catec1/catec1 mutant with modified CaTEC1 alleles. The genes of CaTec1p-bindig proteins will be cloned by a yeast-two-hybrid screen of cDNA libraries, which will be generated from developmentally different cells during the life cycle of C. albicans.

保守的TEA转录因子CaTec1p调控白色念珠菌的形态发生发育和毒力特性。CaTEC1的过表达挽救了几种类型的信号转导突变体的毒力缺陷，并加剧了白色念珠菌野生型菌株的毒力。拟议的研究项目将探讨以下潜在机制：1 . CaTec1p诱导特定目标启动子；CaTec1p的结构-功能关系；CaTec1p与其他调节蛋白的相互作用。catec1p依赖基因的启动子将用于鉴定catec1p结合DNA基序。该基序将在菌丝形成和感染过程中通过体内表达报告系统进一步分析。通过catec1/catec1突变体与修饰的catec1等位基因的功能互补，推断出的CaTec1p蛋白氨基酸序列将被赋予不同的功能。catec1p结合蛋白的基因将通过酵母-双杂交cDNA文库筛选克隆，这些文库将从白色念珠菌生命周期中发育不同的细胞中产生。