Floral repressors in Arabidopsis thaliana

拟南芥中的花抑制因子

• 批准号: 5426547
• 负责人: Professor Dr. Markus Schmid
• 金额: --
• 依托单位: Department of Plant Physiology
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2004
• 资助国家: 德国
• 起止时间: 2003-12-31 至 2007-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5426547?language=en
• 关键词: Floral; repressors; Arabidopsis; thaliana

Floral induction has been studied extensively in Arabidopsis thaliana. Over the last decade, tremendous progress has been made in understanding this important developmental process, and many genes promoting flowering have been isolated. Floral repression, however, is less well understood. Similarly, it has been unclear how output of different floral induction pathways controls the expression of genes that control the identity of individual flowers. We have identified a floral repressor, SCHLAFMÜTZE (SMZ), which encodes an AP2-type transcription factor that acts downstream in the photoperiod pathway. SMZ and the paralogous gene SCHNARCHZAPFEN (SNZ) belong to a clade of six AP2-family members that are potential targets of miR172 micro-RNAs. We will study in detail how transcript accumulation of SMZ and SNZ is regulated, including the potential role of miR172 in this process. Transcriptional targets of SMZ and SNZ will be identified using expression profiling as well as genome-wide identification of SMZ/SNZ bindigs sites. Molecular, biochemical and genomic approaches will be complemented by genetic studies, including mutagenesis screens to identify enhancers and/or suppressors of an SMZ gain-of-function allele. Finally, we will begin to characterize seven additional activation-tagged lines in which flowering is delayed, and which are therefore candidates for identifying additional floral repressors.

拟南芥的成花诱导已被广泛研究。在过去的十年里，在理解这一重要的发育过程方面取得了巨大的进展，许多促进开花的基因已经被分离出来。然而，人们对花的压抑知之甚少。同样，不同的花诱导途径的输出如何控制控制单个花的特性的基因的表达也不清楚。我们已经确定了一个花抑制因子，SCHLAFMätze(SMZ)，它编码一个AP2型转录因子，作用于光周期途径的下游。SMZ和同源基因SCHNARCHZAPFEN(SNZ)属于6个AP2家族成员，它们是miR172微RNA的潜在靶标。我们将详细研究SMZ和SNZ的转录积累是如何调控的，包括miR172在这一过程中的潜在作用。SMZ和SNZ的转录靶标将通过表达谱分析以及SMZ/SNZ结合位点的全基因组鉴定来确定。分子、生化和基因组方法将得到遗传学研究的补充，包括突变筛选，以确定SMZ功能获得等位基因的增强子和/或抑制子。最后，我们将开始鉴定另外七个开花延迟的激活标记品系，因此它们是鉴定其他花抑制因子的候选材料。