海馬セルアセンブリのヘテロ型シナプス可塑性

为了研究海马片培养中活跃突触前输入之间的异突触相互作用，采用狂犬病毒追踪技术鉴定会聚靶向共同CA1突触后神经元的CA3突触前神经元。虽然标记本身是成功的，但发现感染的神经元在几天内退化，因此很难通过电生理学评估会聚连接的功能特性。因此，需要改变战略。为此，考虑使用能更好地保存高度组织的原生连接模式的急性海马切片。特别是，急性切片允许在不需要病毒追踪的情况下采样独立的CA3到CA1输入集。两组非重叠突触前输入的最小刺激显示出高度可变的失败事件，并表明趋同连接之间突触前效能的异质性。初步实验表明，刺激一组输入可以诱导长期可塑性，而这在非刺激输入中产生异突触变化。

In order to examine the heterosynaptic interactions between active presynaptic inputs in hippocampal slice cultures, rabies virus tracing technique was implemented to identify CA3 presynaptic neurons that convergently targeted a common CA1 postsynaptic neuron. Although the labelling itself was successful, infected neurons were found to degenerate over several days and consequently it was difficult to assess the functional properties of the convergent connections by electrophysiology. Therefore, a change of strategy was required. To this end, the use of acute hippocampal slices that better preserved the highly organized native connectivity pattern was considered. In particular, acute slices allowed for sampling of independent sets of CA3 to CA1 inputs without the need for viral tracing. Minimal stimulation of two sets of non-overlapping presynaptic inputs showed highly variable failure events, and indicated the heterogeneity of presynaptic efficacy between convergent connections. Pilot experiments showed that stimulation of one set of inputs could induce long-term plasticity and that this produced heterosynaptic changes in the non-stimulated input.