リプログラミングの論理的制御による神経細胞の作製

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Single-cell RNA sequencing can disentangle highly diverse cell populations, and offers a way to unravel Transcriptional Regulatory Network (TRN) controlling cell-reprogramming. To this end, we generated a pool of 20 neuronal transcription factors (TFs) and sought to distinguish exogenous and endogenous TFs using single-cell RNAseq. Taking advantage of the dissimilarity of nucleotide sequences, we could identify exogenous and endogenous genes in induced neurons, and revealed a highly diverse subpopulation of induced neurons with defined gene signatures. We plan to perform validation experiments in collaboration with BSI/RIKEN. These results will be published within this fiscal year.