ウラギンシジミにおける視覚的種内他個体認識の神経行動学的研究

今年的一个重大进步是在远程显微镜装置上实现的，它有一个分光光度计头和一个侧端口。可以同时记录来自不同焦平面的两幅图像和来自特定区域的光谱。我们使用该装置进行眼睛反射率和荧光、角膜/组织玻片透射率和视紫红质动力学的高光谱成像。光谱学和动态瞳孔激活实验表明，长波紫红质在575 nm处达到峰值。此外，通过分析单个小眼的瞳孔对光谱的刺激，我们发现了五到六簇小眼类型，与三种短波长的受体类别一致。这种方法无疑是研究蝴蝶眼睛的一种很有前途的工具。因此，我们将该方法应用于几种鳞翅目，包括一种skipper和两种hawk蛾。另一个进步是使用led制造光刺激装置。我们开发了一种图像分析方法，使用该设备来估计高光谱图像堆栈中的色素浓度。为了验证该装置作为一种生物医学工具，我们测量了青蛙皮肤中的血氧含量：Pirih博士发表了结果（Belusic等人）。科学代表，6:32012,2016)。我们现在在Sokendai有一个带变形光学和去偏振的双寄存器LED合成器，Pirih博士在他延长逗留的两个月里成功地组装了它。这将是我实验室未来几年强有力的研究工具。

A major advance this year was implemented in the telemicroscope setup, which has a spectrophotometer head and a side port. Two images from different focal planes and a spectrum from a defined region can be recorded simultaneously. We used the setup for hyperspectral imaging of eye reflectance and fluorescence, corneal/ tissue slide transmittance and for rhodopsin dynamics. The spectroscopy and dynamic pupil activation experiments on Curetis acuta revealed that the long wavelength rhodopsin peaks at 575 nm. Furthermore, by analyzing the pupils of individual ommatidia to spectral stimulation, we found five to six clusters of ommatidial types, consistent with three short-wavelength receptor classes. The method is certainly a promising tool for studying butterfly eyes with red eyeshine. We thus applied the method to several lepidopteran species including a skipper and two hawkmoths.Another advance was in building light stimulation device using LEDs. We developed an image analysis method using the device for estimating pigment concentrations in a hyperspectral image stack. To validate the device as a biomedical tool, we measured blood oxygenation in the frog skin: Dr Pirih published the results (Belusic et al. Sci rep, 6:32012, 2016). We now have a two-register LED synthesizer with anamorphic optics and a depolarizer in Sokendai, which Dr Pirih assembled successfully during his extended stay for 2 months. This will be a powerful research tool in my laboratory in coming years.