ウラギンシジミにおける視覚的種内他個体認識の神経行動学的研究

今年的一个重大进步是在远程显微镜设置中实现的，该设置具有分光光度计头和侧端口。可以同时记录来自不同焦平面的两个图像和来自定义区域的光谱。我们使用的设置高光谱成像的眼睛反射率和荧光，角膜/组织载玻片透射率和视紫红质动力学。光谱学和动态瞳孔激活实验表明，长波长的视紫红质峰位于575 nm处。此外，通过分析单个小眼的瞳孔光谱刺激，我们发现五到六个小眼类型的集群，符合三个短波长受体类。该方法无疑是一种很有前途的工具，研究蝴蝶的眼睛与红色eyeshine。因此，我们将这种方法应用于几种鳞翅目昆虫，包括一种尺蠖和两种天蛾。另一个进展是利用LED构建光刺激装置。我们开发了一种图像分析方法，使用该设备来估计高光谱图像堆栈中的色素浓度。为了验证该设备作为生物医学工具的有效性，我们测量了青蛙皮肤中的血氧含量：Pirih博士发表了结果（Belusic et al. Sci rep，6：32012，2016）。我们现在有一个双寄存器LED合成器与变形光学和消偏器在Sokendai，这是Pirih博士在他延长逗留2个月期间成功组装的。这将是未来几年我实验室的一个强大的研究工具。

A major advance this year was implemented in the telemicroscope setup, which has a spectrophotometer head and a side port. Two images from different focal planes and a spectrum from a defined region can be recorded simultaneously. We used the setup for hyperspectral imaging of eye reflectance and fluorescence, corneal/ tissue slide transmittance and for rhodopsin dynamics. The spectroscopy and dynamic pupil activation experiments on Curetis acuta revealed that the long wavelength rhodopsin peaks at 575 nm. Furthermore, by analyzing the pupils of individual ommatidia to spectral stimulation, we found five to six clusters of ommatidial types, consistent with three short-wavelength receptor classes. The method is certainly a promising tool for studying butterfly eyes with red eyeshine. We thus applied the method to several lepidopteran species including a skipper and two hawkmoths.Another advance was in building light stimulation device using LEDs. We developed an image analysis method using the device for estimating pigment concentrations in a hyperspectral image stack. To validate the device as a biomedical tool, we measured blood oxygenation in the frog skin: Dr Pirih published the results (Belusic et al. Sci rep, 6:32012, 2016). We now have a two-register LED synthesizer with anamorphic optics and a depolarizer in Sokendai, which Dr Pirih assembled successfully during his extended stay for 2 months. This will be a powerful research tool in my laboratory in coming years.