Functional analysis of intergenic regions by contiguous introduction of genomic deletion mutations

通过连续引入基因组缺失突变对基因间区域进行功能分析

• 批准号: 15H04284
• 负责人: KOKUBU CHIKARA
• 金额: $ 10.9万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2015
• 资助国家: 日本
• 起止时间: 2015-04-01 至 2018-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15H04284/
• 关键词: ゲノム工学; 疾患モデル; 遺伝学; ゲノム; ゲノム編集

项目成果

Report on the Conference on Transposition and Genome Engineering 2015 (TGE 2015): advancing cutting-edge genomics technology in the ancient city of Nara.

2015 年转座与基因组工程会议 (TGE 2015) 报告：在古城奈良推进尖端基因组学技术。

• DOI: 10.1111/gtc.12367
• 发表时间: 2016
• 期刊: Genes Cells.
• 作者: Woltjen K;Yamamoto T;Kokubu C;Takeda J.
• 通讯作者: Takeda J.

Homozygous mutant mouse embryonic stem cell bank arising from autodiploidization during haploid gene-trap mutagenesis

单倍体基因诱变过程中自二倍化产生的纯合突变小鼠胚胎干细胞库

• 发表时间: 2015
• 作者: Yamanishi A.;Kondo R.;Matsuba A.;Tokunaga M.;Kokubu C.;Ishida Y. and Takeda J.
• 通讯作者: Ishida Y. and Takeda J.

Collection of homozygous mutant mouse embryonic stem cells arising from autodiploidization during haploid gene trap mutagenesis.

• DOI: 10.1093/nar/gky183
• 发表时间: 2018-06-01
• 期刊: Nucleic acids research
• 影响因子: 14.9
• 作者: Yamanishi A;Matsuba A;Kondo R;Akamatsu R;Tanaka S;Tokunaga M;Horie K;Kokubu C;Ishida Y;Takeda J
• 通讯作者: Takeda J

A combination of Bloom gene inactivation and CRISPR/Cas9-mediated DNA cleavage may facilitate construction of a loss-of-heterozygosity library of the mouse genome

Bloom 基因失活和 CRISPR/Cas9 介导的 DNA 切割相结合可能有助于构建小鼠基因组杂合性缺失文库

• 发表时间: 2015
• 作者: Kamitani T.;Yamanishi A.;Yoshimura Y.;Kokubu C. and Takeda J.
• 通讯作者: Kokubu C. and Takeda J.