遺伝子発現に対するＲＮＡ分解および転写後制御の寄与

最初的建议是确定RNA衰变在介导最终RNA丰度变化中的潜在作用。在上一财年，我进一步完善了BRIC seq的RNA衰变图谱，进一步分类了能够通过RNA衰变上调的有趣的一组基因，以及获得正反馈的基因，它们往往是转录因子。我开始进行验证，以确认RNA衰变是驱动基因表达变化的主要因素。此外，我已经覆盖了转录因子数据，以阐明转录因子反馈的可能性。此外，我比较了HIF-1 ChIP seq数据，发现HIF-1和RNA衰变在调控上似乎是不同的。除了实验室实验外，我还综合了这些发现，准备在学术期刊上发表一篇论文。

The original proposal was to identify the potential role of RNA decay in mediating changes of the eventual RNA abundance.In the last fiscal year, I was to further refine the RNA decay profile of BRIC seq, further classified the interesting set of genes that were able to be up-regulated via RNA decay, and genes that receive positive feedback and they tend to be transcription factors. I started to conduct validations to confirm that the RNA decay is the main factor that drive changes in gene expression.In addition I have overlaid the transcription factor data to elucidate the possibility of feedback for the transcription factors.In addition, I have compared the HIF-1 ChIP seq data to find that HIF-1 and RNA decay seems to be distinct in regulation. Alongside the lab experiments, I have been synthesizing the findings to publish a paper in an academic journal.