イメージングと時系列統計解析による細胞死制御機構の定量的な解析

我成功地应用活细胞成像系统，在单个细胞中同时显示了应激激活的激酶JNK和p38的活动。使用这个系统，我发现了JNK活性在不同压力下的细胞间变化。我发现了与p38激酶有关的调节机制，这导致了JNK活性的异质性。我的数据表明，JNK活性的异质性决定了细胞在应对严重压力(如紫外线)时的命运决定。对p38和JNK活性动态的统计分析确定了可以预测细胞死亡的参数。这项工作在一篇论文中进行了总结，并提交给了一份同行评议的期刊。总而言之，根据最初的计划，我取得了很好的进展。

I successfully applied a live cell imaging system to visualize the activities of the stress-activated kinases JNK and p38 simultaneously in single cells. Using this system, I discovered cell-to-cell variability of JNK activity in response to various stresses. I found regulatory mechanisms involving p38 kinase which generated the heterogeneity in JNK activity. My data indicate that the heterogeneity in JNK activity determines cell fate decisions in response to severe stresses, such as UV. Statistical analysis of p38 and JNK activity dynamics identified parameters which were predictive for cell death. The work was summarized in a paper and submitted to a peer-reviewed journal. In summary, I made good progress according to the initial plans.