ヤブコウジ科における葉粒菌垂直伝播の系統学的研究

伞形科植物叶杆菌垂直传播的系统发育研究

• 批准号: 16J12040
• 负责人: BINTI JULIUS AVELINAH
• 金额: $ 1.22万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2016
• 资助国家: 日本
• 起止时间: 2016-04-22 至 2018-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16J12040/
• 关键词: Ardisia; Amblyanthopsis; Amblyanthus; Burkholderia; Co-phylogenetic; leaf-nodule bacteria; Symbiosis; Primulaceae; Leaf nodule bacteria; Phylogenetic; Systematic

Bacterial leaf-nodule symbiosis has been hypothesized evolved only once in Myrsinoideae but this hypothesis is partly confirmed because emphasizing only on Ardisia (§Crispardisia). This study tested further evolution of leaf nodulation within Myrsinoideae by including three nodulated genera and determine the bacterial symbionts that colonized Amblyanthus and Amblyanthopsis. Host-symbiont cophylogenetic analysis also conducted. Phylogenetic tree for both host and endosymbionts reconstructed using Bayesian inferences and maximum likelihood in separate and/or combined dataset analyses. The phylogeny of host (61 samples) and the endosymbionts (167 samples) are based on nrITS, rpL32-trnL, trnS-trnG and recA and gyrB, respectively. Host-symbiont cophylogenetic analysis was conducted using JANE. The presence of bacterial symbionts was tested on 24 nodulated and 3 non-nodulated taxa.The host nodulated genera and their endosymbionts were not monophyletic and formed into two groups (A) Amblyanthus and (B) §Crispardisia + Amblyanthopsis. Comparison tree shows incongruence topologies between the host and endosymbionts. Cophylogenetic analysis revealed that the diversification of endosymbionts is mainly by the duplication and host switching. The housekeeping genes amplified from the leaf nodulating symbionts suggested they form a clade within Burkholderia. PCR detection confirmed the present of bacterial symbionts only in nodule part and the seeds and restricted only to nodulated taxa. Overall, this study revealed that the leaf nodulation evolved twice and originated independently.

细菌叶结共生在桃金猴科中只进化了一次，但由于只强调了Ardisia（§Crispardisia），这一假设得到了部分证实。本研究通过纳入3个结瘤属，进一步测试了桃科植物叶片结瘤的进化，并确定了定植在Amblyanthus和Amblyanthopsis上的细菌共生体。并进行了宿主-共生体的共同发育分析。利用贝叶斯推断和最大似然在单独和/或组合数据集分析中重建宿主和内共生体的系统发育树。宿主（61个样本）和内生共生体（167个样本）的系统发育分别基于nrITS、rpL32-trnL、trnS-trnG和recA和gyrB。采用JANE进行宿主-共生体共进化分析。在24个结瘤类群和3个非结瘤类群上检测了细菌共生体的存在。寄主结瘤属及其内共生体不是单系的，分为两组(A) Amblyanthus和(B)§Crispardisia + Amblyanthopsis。比较树显示宿主和内共生体之间的拓扑结构不一致。共同进化分析表明，内共生体的多样化主要是通过复制和寄主切换。从结叶共生体中扩增出的管家基因表明，它们在伯克霍尔德菌中形成了一个分支。PCR检测证实，细菌共生体仅存在于根瘤部分和种子中，且仅限于根瘤类群。总的来说，本研究揭示了叶片结瘤的两次进化和独立起源。

项目成果

Generic limits of Ardisia (Primulaceae-Myrsinoideae) in Tropical Asia

热带亚洲紫金牛属（报春花科-紫金牛科）的属限

• 发表时间: 2016
• 作者: AVELINAH JULIUS;TIMOTHY M.A. UTTERIDGE;JOSE SAID GUTIRREZ-ORTEGA,TADASHI KAJITA
• 通讯作者: JOSE SAID GUTIRREZ-ORTEGA,TADASHI KAJITA

Royal Botanic Gardens, Kew(United Kingdom)

邱园皇家植物园（英国）

Progress in Malesian Primulaceae: moving on from ‘Myrsinaceae’. An oral presentation during FM10 in RBGE

马来报春花科进展：从“紫金牛科”开始，在 RBGE FM10 期间进行口头报告。

• 发表时间: 2016
• 作者: TIMOTHY M.A. UTTERIDGE;CLARE DRINKELL;ANNE DUEBERNUS;SUZANA SABRAN;AVELINAH JULIUS
• 通讯作者: AVELINAH JULIUS

Ardisia gasingoides (Primulaceae-Myrsinoideae), a new species from southern Peninsular Malaysia

• DOI: 10.11646/phytotaxa.291.4.5
• 发表时间: 2017-01-18
• 期刊: PHYTOTAXA
• 影响因子: 1.1
• 作者: Julius, Avelinah;Kajita, Tadashi;Utteridge, Timothy M. A.
• 通讯作者: Utteridge, Timothy M. A.

Lysimachia kraduengensis (Primulaceae), a new species from northeastern Thailand

• DOI: 10.11646/phytotaxa.289.1.5
• 发表时间: 2016-12-20
• 期刊: PHYTOTAXA
• 影响因子: 1.1
• 作者: Julius, Avelinah;Tagane, Shuichiro;Utteridge, Timothy
• 通讯作者: Utteridge, Timothy