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廃用性筋萎縮からの回復でおこる炎症に対する効果的治療法開発に向けた基礎研究

基础研究旨在开发有效治疗废用性肌肉萎缩恢复引起的炎症的方法

基本信息

批准号：
17K01488
负责人：
廣島玲子
金额：
$ 3.08万
依托单位：
Kansai University of Welfare Sciences
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2017
资助国家：
日本
起止时间：
2017-04-01 至 2024-03-31
项目状态：
已结题

项目摘要

廃用性筋萎縮は医療臨床現場では頻繁に起こり、リハビリテーション分野では筋萎縮からの回復は重要課題となる。先行研究で廃用性筋萎縮を発症した骨格筋は脆弱となり自己体重を負荷するだけで筋損傷が起こり、そこから炎症反応が惹起され、その後回復過程へと進んでいくことが明らかになった。本研究は、廃用性筋萎縮が原因で起こる損傷や炎症が回復過程に及ぼす影響について筋細胞レベル、分子レベル、遺伝子レベルで詳細に分析し、またその回復過程において医療現場で用いられるリハビリテーション治療法介入の効果やその効率について検討することを目的とする。研究方法として、Wistar系雄ラットを使用し下肢にギプス固定を施し、ヒラメ筋に廃用性筋萎縮を発生させる。2週後ギプス固定を解放し、ラットは再びケージ内を自由に動くことができる。ギプス固定解放直後に抗炎症剤をラットヒラメ筋に注射し、その後の回復過程を3、7、14日後に検討する注射群と、抗炎症剤注射なしで自然治癒3，7，14日後の対照群を比較・検討する。本実験指標として、ミオシン重鎖アイソフォーム3種(MHC-Ⅰβ、MHC-Ⅱa、MHC-Ⅱb)、炎症性サイトカイン3種(IL-6、IL-1、TNF-α)のmRNA遺伝子およびタンパク質レベルでの発現量を分析する。本実験では主として炎症実験(抗炎症剤投与による影響の検討)を実施し、廃用性筋萎縮からの早期回復段階で起こる炎症の程度やメカニズム解明への足掛かりを検証する。その後、炎症実験の結果を踏まえ数種のリハビリテーション治療法を介入しそれらの効果や効率性について比較・検討する。本研究結果を基に、どのような治療方法が最も有効か、またそれら治療介入の開始時期や期間の最適化などを臨床現場に提案していく。

In the field of sexual malaise and medical treatment, there are many important problems in the field of medical treatment. First of all, we should study the symptoms of sexual muscular dystrophy, skeletal tendons, fragile tendons, your weight, your weight, your tendons, your muscles, your weight, your weight, your muscles, your muscles, your weight, your weight, your muscles, your muscles, your weight, your weight, your muscles, your muscles, your weight, your weight, your muscles, your muscles, your weight, your weight, your muscles, your muscles, your weight, your weight, your muscles, your weight, your weight, your muscles, your muscles, your weight, your muscles, your muscles, your weight, your weight, your muscles, your muscles, your In this study, the causes of sexual myasthenia gravis were related to the process of inflammation and the effects of inflammation on the process of inflammation, including cellular analysis, molecular analysis, molecular analysis, and clinical treatment. The research methods were as follows: the lower limbs were fixed, the lower limbs were fixed, the muscles were fixed, the sexual tendons were atrophied, and the sexual tendons were atrophied. Wistar was used in the study. In the future, you will be free to move freely, and then you will be free to do so. The anti-inflammatory drugs were injected directly after the liberation, and the injection group was injected after the procedure of 3, 7 and 14 days, and the anti-inflammatory group was injected into the natural treatment group after 14 days. This article refers to three types of severe acute respiratory syndrome (MHC- Ⅰ β, MHC- Ⅱ a, MHC- Ⅱ b), and three inflammatory diseases (IL-6, IL-1, TNF- α). The main purpose of this study is to evaluate the degree of inflammation (anti-inflammation and anti-inflammation) in the early stage of treatment. After treatment, the results of inflammation test showed that several kinds of treatment methods were used to treat the disease, and the results were compared with those in the control group. The results of this study are as follows: the most effective methods for the treatment of chronic hepatitis, the most effective and the most effective in the period at the beginning of the period of intervention.