Investigation of the function of zinc in the cellular signal transduction of monocytes

锌在单核细胞信号转导中的功能研究

• 批准号: 5448401
• 负责人: Professor Dr. Hajo Haase
• 金额: --
• 依托单位: Fachgebiet Lebensmittelchemie und Toxikologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2005
• 资助国家: 德国
• 起止时间: 2004-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5448401?language=en
• 关键词: Investigation; function; zinc; cellular; signal

Zinc is an essential trace element for the immune system. Our results indicate that, among other functions, zinc ions are a component of monocytic signal transduction. During the previous funding period we analyzed the role of zinc signals in pathogeninduced signaling. In the present application we intend to investigate the function of zinc signals for the differentiation of monocytic precursors. Preliminary data show that differentiation is accompanied by a decrease in free zinc and that a further reduction of free intracellular zinc by chelators strongly augments monocytic differentiation. We will investigate how changes in the zinc homeostasis of these cells contribute to the differentiation process and characterize the molecular events in the cell that are regulated by zinc. Furthermore, we will examine if zinc is a specific trigger for monocyte formation, or promotes differentiation of the common precursor cells into other cell types, especially granulocytes, as well. Finally, by investigating additional parameters of monocyte function we will analyze the effects of zinc on the functionality of the monocytes and the biological relevance of the zinc status for monocytic differentiation from primary human haematopoietic stem cells.

锌是免疫系统必需的微量元素。我们的研究结果表明，除其他功能外，锌离子是单核细胞信号转导的一个组成部分。在上一个资助期间，我们分析了锌信号在病原体诱导的信号传导中的作用。在本申请中，我们打算研究锌信号对于单核细胞前体分化的功能。初步数据显示，分化伴随着游离锌的减少，并且通过螯合剂进一步减少游离细胞内锌强烈增强单核细胞分化。我们将研究这些细胞的锌稳态的变化如何有助于分化过程，并表征锌调控的细胞中的分子事件。此外，我们将研究锌是否是单核细胞形成的特异性触发剂，或者是否促进常见前体细胞分化为其他细胞类型，特别是粒细胞。最后，通过研究单核细胞功能的其他参数，我们将分析锌对单核细胞功能的影响，以及锌状态对单核细胞从原代人造血干细胞分化的生物学相关性。