Decomposition of sulfides and carbonaceous matters in double refractory gold ores by fungal-released enzymes

真菌释放酶分解双难熔金矿石中的硫化物和含碳物质

• 批准号: 18J10835
• 负责人: KONADU KOJO Twum
• 金额: $ 1.22万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2018
• 资助国家: 日本
• 起止时间: 2018-04-25 至 2020-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18J10835/
• 关键词: Gold recovery; Carbonaceous gold ore; Enzymes; Acidianus brierleyi; QEMSCAN; Phanerochaete chrysosporium; Peroxidase enzymes

Double refractory gold ore containing gold-bearing sulfides and carbonaceous matter was sequentially treated with iron and sulfur oxidising archaea Acidianus brierleyi and lignin-degrading enzymes from Phanerochaete chrysosporium. This treatment resulted in an increase in gold recovery from 24% to 92%. Biooxidation of the sulfide minerals is already known, and therefore, the secondary focus, after improving the gold recovery, was characterising the carbonaceous matter. The transformation of the carbonaceous matter during the sequential treatment was investigated using QEMSCAN analysis, Raman and 3D fluorescence spectroscopy. The results show that the carbonaceous matter was initially hosted in illite, a silicate mineral, and the amount of carbon to illite affected the texture of the carbonaceous illite mineral. The enzymatic decomposition of the carbonaceous matter was found to be accelerated if the sample had undergone a prior treatment to decompose sulfide minerals. This is because the enzymes are susceptible to arsenic poisoning and therefore, the oxidative dissolution of arsenopyrite aided the enzymatic reaction. The lignin-degrading enzymes preferentially attacked the defects in the graphitic structure of carbonaceous matter and in the process, produced humic-like substances. The humic-like substances that are produced by the enzyme treatment acted as one of the binding agents in the agglomeration of the carbonaceous aluminosilicate residue (C-Si-Al). This new C-Si-Al was partially removed by alkaline washing step to improve gold recovery from DAC from 76% to 92%.

采用铁硫氧化古菌Acidianus brierleyi和黄孢原毛革菌木质素降解酶对含金硫化物和碳质双重难处理金矿石进行了处理。这种处理导致金回收率从24%提高到92%。硫化物矿物的生物氧化是已知的，因此，在提高金回收率之后，第二个焦点是表征碳质物质。利用QEMSCAN分析、拉曼光谱和三维荧光光谱研究了连续处理过程中碳质物质的转化。结果表明，碳质物质最初以伊利石（一种硅酸盐矿物）的形式存在，伊利石中碳的含量影响碳质伊利石矿物的结构;如果样品经过预处理以分解硫化物矿物，则碳质物质的酶解速度会加快。这是因为酶对砷中毒敏感，因此，毒砂的氧化溶解有助于酶反应。木质素降解酶优先攻击碳质物质石墨结构中的缺陷，并在此过程中产生类腐殖酸物质。酶处理产生的类腐殖物质在碳质硅铝酸盐残渣（C-Si-Al）的团聚过程中起着粘结剂的作用。这种新的C-Si-Al通过碱洗步骤被部分去除，以将从DAC的金回收率从76%提高到92%。

项目成果

Eff ect of carbonaceous matter on bioleaching of Cu from carbonaceous chalcopyrite ore

碳质物质对碳质黄铜矿矿石生物浸出铜的影响

• 发表时间: 2019
• 作者: Konadu;K.T.;Sakai;R.;Flores;D.;M.;M.;Harrison;S.T.;Osseo-Asare;K.;Sasaki;K.
• 通讯作者: K.

Sequential biotreatment of carbonaceous silva ore

碳质林矿的序贯生物处理

• 发表时间: 2019
• 作者: Flores;D.;M.;M.;Konadu;K.;T.;Sakai;R.;Sasaki;K.,
• 通讯作者: K.,

Bio-mineral processing of double refractory gold ore in sequential treatment using a thermophilic archaeon followed by fungal spent medium

使用嗜热古菌和真菌废培养基连续处理双难熔金矿石的生物矿物加工

• 发表时间: 2019
• 作者: Kojo T. Konadu;Susan T. L. Harrison;Kwadwo Osseo-Asare;Keiko Sasaki
• 通讯作者: Keiko Sasaki

Sequential pretreatment of double refractory gold ore (DRGO) with a thermophilic iron oxidizing archeaon and fungal crude enzymes

• DOI: 10.1016/j.mineng.2019.04.043
• 发表时间: 2019-07
• 期刊: Minerals Engineering
• 影响因子: 4.8
• 作者: Kojo T. Konadu;R. Huddy;S. Harrison;K. Osseo-Asare;K. Osseo-Asare;K. Sasaki

Effect of washing on the sequential bio-oxidation of carbonaceous matters and sulfides in double refractory gold ore (DRGO) by white-rot fungus Phanerochaete chrysosporium and thermophilic archearon Acidianus brierleyi

洗选对白腐真菌黄孢原平毛革菌和嗜热古菌布氏酸菌顺序生物氧化双难熔金矿石（DRGO）中碳质和硫化物的影响

• 发表时间: 2018
• 期刊: International Symposium on Earth Science and Technology
• 作者: Konadu;K.T.;Pahlevi;N.D.;Harrison;S.T.L;K.;Sasaki;K.
• 通讯作者: K.