膵再生医療に向けた立体膵組織の作製技術の確立

本研究的目的是构建生物人工胰岛并优化其培养条件。本研究的第一个重点是利用可透氧PDMS芯片制备胰腺球形聚集体（球状体），其在长时间培养（1周）期间为细胞提供足够的氧合并防止缺氧核心的发展。下一个关键点是在培养基中使用抗氧化剂抗坏血酸-2-磷酸，其保护细胞免受由于体外条件与体内最佳条件不同而产生的活性氧物质的影响。第三个关键点是培养基中的葡萄糖浓度。虽然单层细胞培养在高葡萄糖中，但我的研究表明，较低的葡萄糖浓度有利于球状体的培养。

The goal of my research was to fabricate bioartificial pancreatic islets and optimise their culture conditions. The first key point of my work is the use of oxygen permeable PDMS-chip to fabricate pancreatic spherical aggregates (spheroids), which provided cells with adequate oxygenation and prevented development of hypoxic core during long culture (1 week).The next key point is the use of the antioxidant ascorbic acid-2-phosphate in culture medium, which protects cells from reactive oxygen species generated due to the fact that in vitro conditions differ from the optimal in vivo ones.The third key point is the glucose concentration in culture medium. Although monolayer is culture in high glucose, my investigation showed that lower glucose concentration is beneficial for spheroid culture.