SPP 1028: Molekulare Differenzierungsmechanismen von Epithelien

SPP 1028：上皮细胞的分子分化机制

• 批准号: 5467802
• 金额: --
• 依托单位: Forschungsschwerpunkt Zell- und Tumorbiologie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 1997
• 资助国家: 德国
• 起止时间: 1996-12-31 至 2002-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5467802?language=en
• 关键词: SPP; 1028; Molekulare; Differenzierungsmechanismen; von

Structure and function of epithelial organs, such as skin, mucosa, lung, and the digestive tract are produced and maintained by tissue specific differentiated cell types. Both the permanently self-renewing epithelia as well as their supporting connective tissues are in a dynamic process of continuous growth, reorganization and differentiation. These processes involve and are controlled by both tissue-resident and infiltrating cells. The mechanisms controlling these complex processes are only partially understood. It is known that complex interactions between epithelial, mesenchymal and neuroectodermal cells are involved mediated by diffusible factors as well as by direct cell-cell contact. In view of the complexity of these mechanisms, it appeared reasonable to coordinate the work of different groups with expertise in studying differentiation of isolated cells as well as epithelial organs in a research program to study basic mechanisms of tissue interactions controlling epithelial differentiation. The specific tasks of this research program shall focus on the following subjects:1. Production, release and mechanism of action of diffusible factors regulating epithelial differentiation,2. Analysis and characterization of the changes produced by these factors in target cells on transcription, translation level of target genes,3. Analysis of regulatory mechanisms based on cell-cell interactions in experimental models, such as conventional and organotypic cocultures, organ cultures and seclected animal models.

上皮器官（例如皮肤、粘膜、肺和消化道）的结构和功能是由组织特异性分化细胞类型产生和维持的。永久自我更新的上皮细胞及其支持结缔组织都处于持续生长、重组和分化的动态过程中。这些过程涉及组织驻留细胞和浸润细胞并受其控制。控制这些复杂过程的机制仅被部分了解。众所周知，上皮细胞、间充质细胞和神经外胚层细胞之间复杂的相互作用涉及由扩散因子以及直接细胞与细胞接触介导的。鉴于这些机制的复杂性，在研究控制上皮分化的组织相互作用的基本机制的研究计划中，协调具有研究分离细胞和上皮器官分化专业知识的不同小组的工作似乎是合理的。本研究计划的具体任务主要包括以下几个方面： 1．调节上皮分化的扩散因子的产生、释放及其作用机制；2．分析和表征这些因素在靶细胞中对靶基因转录、翻译水平产生的变化；3．基于实验模型中细胞与细胞相互作用的调节机制分析，例如传统和器官型共培养、器官培养和选定的动物模型。