SPP 1128: Optical Analysis of the Structure and Dynamics of Supra Molecular Biological Complexes

该计划的目标是生物大分子超分子复合物的三维结构和动力学的定量光学分析和理论建模。这些“分子机器”应特别在相关细胞结构的背景下进行检查，因为它们对许多细胞功能具有根本重要性。感兴趣的中心是定量的最高分辨率的光光学分析方法，与特定的荧光标记方法和定量方法的解释，通过使用建模和模拟。除了这些分析方法外，我们还对超微结构的互补分析进行了研究。膜相关复合物和细胞质复合物，例如：光学分析和外聚体复合物的建模;中心体的结构分析。突触细胞基质的发生和功能，例如蛋白质和蛋白质复合物进入突触的高分辨率分析。3.活性染色质复合物，例如，特定人类基因区域的纳米结构的光学分析和建模;活细胞中染色质重塑复合物的光学分析。它旨在本地建立高分辨率光学程序，荧光信息程序和光学纳米显微镜的方法，并使其可用于支持研究。

The goal of the programme is the quantitative optical analysis and theoretical modelling of the three dimensional structure and dynamics of supra molecular complexes of biological macromolecules. These "molecular machines" shall be examined especially in the context of the relevant cellular structures as they are of fundamental importance for numerous cellular functions. In the centre of interest are quantitative highest resolution light optical methods of analysis in connection with specific fluorescence labelling methods and quantitative approaches of interpretation by the use of modelling and simulation. Together with these methods of analysis complementary analysis of ultra-structures are appreciated.The interdisciplinary works is concentrated on the following topics:1. Membrane associated and cytoplasmatic complexes e.g. the optical analysis and modelling of coatameric complexes; structure analysis of the centrosome.2. Genesis and function of the synaptic cytomatrix, e.g. high resolution analysis of transport of proteins and protein complexes into the synapses.3. Active chromatin complexes e.g. optical analysis and modelling of the nanostructure of specific human gene regions; optical analysis of chromatin remodelling complexes in living cells.It is intended to locally establish methods for high resolution light optical procedures, fluorescence information procedures, and light optical nanoscopy, and make them available to support the investigations.