SPP 1128: Optical Analysis of the Structure and Dynamics of Supra Molecular Biological Complexes

该计划的目标是生物大分子的超分子复合物的三维结构和动力学的定量光学分析和理论建模。这些“分子机器”应该在相关细胞结构的背景下进行研究，因为它们对许多细胞功能至关重要。在感兴趣的中心是定量的最高分辨率的光学分析方法与特定的荧光标记方法和定量的解释方法，通过使用建模和模拟。与这些分析方法一起，对超结构的补充分析是值得赞赏的。跨学科的工作主要集中在以下几个方面：1。膜相关复合物和细胞质复合物，例如膜复合物的光学分析和建模；中心体的结构分析突触细胞基质的发生和功能，例如蛋白质和蛋白质复合物进入突触的高分辨率分析。活性染色质复合物，例如特定人类基因区域的光学分析和纳米结构建模；活细胞中染色质重塑复合物的光学分析。目的是在当地建立高分辨率光光学程序、荧光信息程序和光光学纳米显微镜的方法，并使它们可用于支持研究。

The goal of the programme is the quantitative optical analysis and theoretical modelling of the three dimensional structure and dynamics of supra molecular complexes of biological macromolecules. These "molecular machines" shall be examined especially in the context of the relevant cellular structures as they are of fundamental importance for numerous cellular functions. In the centre of interest are quantitative highest resolution light optical methods of analysis in connection with specific fluorescence labelling methods and quantitative approaches of interpretation by the use of modelling and simulation. Together with these methods of analysis complementary analysis of ultra-structures are appreciated.The interdisciplinary works is concentrated on the following topics:1. Membrane associated and cytoplasmatic complexes e.g. the optical analysis and modelling of coatameric complexes; structure analysis of the centrosome.2. Genesis and function of the synaptic cytomatrix, e.g. high resolution analysis of transport of proteins and protein complexes into the synapses.3. Active chromatin complexes e.g. optical analysis and modelling of the nanostructure of specific human gene regions; optical analysis of chromatin remodelling complexes in living cells.It is intended to locally establish methods for high resolution light optical procedures, fluorescence information procedures, and light optical nanoscopy, and make them available to support the investigations.