Effect of increased paternal age on mitochondria of embryos

父亲年龄增加对胚胎线粒体的影响

• 批准号: 22K05959
• 负责人: 岩田 尚孝
• 金额: $ 2.66万
• 依托单位: Tokyo University of Agriculture
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K05959/
• 关键词: 加齢; 父親; 胚; ミトコンドリア; テロメア長

同一の雄マウス個体(C57/B6)を使い、若齢時と加齢時に若齢メスマウスと交配し得た胚とその産子を比較した。ミトコンドリアのDNAコピー数とテロメア長はリアルタイムPCRで測定した。体内から回収した胚ではウシの加齢に伴い胚の発生率は変わらないが、胚の持つミトコンドリアDNAコピー数が減りテロメアの長さが有意に短くなることが明らかになった。2細胞から回収し体外で胚盤胞期胚まで発育した胚を比較すると、雄の加齢により細胞数が減少するなど胚の発育率が低下し、体内と同様にミトンドリア数が減ることが明らかになった。胚盤胞期胚ではミトコンドリアDNAコピー数とテロメア長の間には有意に相関が認められた。この相関はウシの体外発育胚やブタの単為発生胚でも認められた。雄の若齢時と加齢に作成した産子を生後直ちに実験に供し、体重を比較すると、加齢に伴い産子体重が減少することが明らかになった。さらにその心臓の細胞に含有されるミトコンドリア数が有意に減少した。雄の産子ではテロメアの長さも有意に減少したがメスの産子では差が認められなかった。メス産子のテロメア長やミトコンドリア数は雄産子よりも多いことが分かった。これらのことから、マウスでは雄の加齢は胚や産子のミトコンドリア数やテロメア長に影響する事が明らかになった。またミトコンドリア数とテロメア長の間の相関は動物種で保存されている現象ではないかと考えられた。若齢と加齢時の雄マウスから得た胚盤胞期胚をRNAseqに供し変化の分子背景を得ることが出来た。

The same male parents (C57/B6) make babies, and if they try to mate at the same time, they will get a good match. We need to check the number of DNA. We need to determine the number of PCR measurements. In vivo, the birth rate of embryos was increased, the birth rate of embryos was increased, and the birth rate of embryos was increased. The birth rate of embryos was increased. The birth rate of embryos was significantly higher than that of embryos. The birth rate of embryos was significantly higher than that of embryos. The birth rate of embryos was significantly higher than that of embryos. The birth rate of embryos was significantly higher than that of DNA. 2) in vitro, the embryo was incubated in the cell stage, the embryo was cultured, the male was added, the cell number was less, the embryo fertility rate was low, and the same embryo in vivo was used to count the embryos. In the stage of embryo development, the number of embryos in the cell stage of the embryo, the DNA, the mother, the mother, the The embryo was cultured in vitro. The embryo was born and the embryo was born. If you add a baby to make a baby after birth, your weight is lower than that of your baby, and your weight is low. There is an intentional decrease in the number of cells in the heart. The male child is in a bad mood. He wants to tell him that he has no intention of making a mistake. Please tell me how to count the number of male children. Please tell me that you are not in a good mood, and that you are not in a position to know what is going on. We need to know how many times we need to make sure that the animal species are saved as if they were used. If the RNAseq of the cell stage embryos is obtained during the incubation period, the molecular background of the embryos will be affected by the molecular background.