Rewiring the brain: remote-controlled axon guidance by magnetic nanoparticles to improve Parkinson's therapies

重新布线大脑：通过磁性纳米粒子远程控制轴突引导以改善帕金森氏症的治疗

• 批准号: 22K06430
• 负责人: Raudzus Fabian
• 金额: $ 2.58万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K06430/
• 关键词: Dopaminergic neurons; Stem cells (iPSCs); Magnetic nanoparticles; Halbach magnet; Axon guidance; CNS regeneration; Parkinson's disease; Nanotechnology; Cell Replacement Therapy; Parkinson's Disease; Magnetic Nanoparticles; Pluripotent Stem Cell

Within the first year of the grant, various important steps were performed that serve as the prerequisite and basis for the project. 1) Suitable magnetic nanoparticles were acquired. Two types of nanoparticles turned out to be tolerated by the cells without any notable side effects. 2) The magnetic applicator was designed and built. The first 3D-printed version was unfortunately not successful. Therefore, two devices from aluminum were constructed. Now, these devices have an improved and more homogenous magnetic field. 3) The application of nanoparticles to dopaminergic progenitors was established and optimized. The most difficult part of the attachment of spheres is finally solved. 4) First magnetic manipulations are performed, and the optimization of different parameters is ongoing.

在赠款的第一年内，执行了作为项目前提和基础的各种重要步骤。1)获得合适的磁性纳米颗粒。两种类型的纳米颗粒被证明是细胞耐受的，没有任何明显的副作用。2)设计并制造了磁性施用器。第一个3D打印版本并不成功。因此，构建了两个铝制器械。现在，这些设备具有改进的和更均匀的磁场。3)建立并优化了纳米颗粒对多巴胺能祖细胞的应用。最困难的部分的附着球终于解决了。4)首先进行磁操作，并进行不同参数的优化。

项目成果

Universita' Degli Studi Di Pisa(イタリア)

比萨大学（意大利）

Johannes Gutenberg University Mainz(ドイツ)

美因茨约翰内斯古腾堡大学（德国）

Spatiotemporal control of RAS pathway activation by biofunctionalized nanoparticles for guided neurite growth, maturation, and regeneration

通过生物功能化纳米粒子对 RAS 通路激活的时空控制，以引导神经突生长、成熟和再生

• 发表时间: 2022
• 作者: Fabian Raudzus (presenter);Hendrik Schoeneborn;Sebastian Neumann;Hiromasa Adachi;Emilie Secret;Oliver Brylski;Christine Menager;Jean-Michel Siaugue;Rolf Heumann;Jun Takahashi
• 通讯作者: Jun Takahashi