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腸内細菌叢による大腸癌subtypingとその免疫微小環境のscRNAseq解析

肠道菌群及其免疫微环境对结直肠癌亚型的 scRNAseq 分析

基本信息

批准号：
22K07283
负责人：
永吉絹子
金额：
$ 2.66万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2022
资助国家：
日本
起止时间：
2022-04-01 至 2025-03-31
项目状态：
未结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K07283/
关键词：
microbiome 大腸癌 CMS分類免疫微小環境 scRNA-seq CMS 腸内細菌叢 single cell RNA sequence

项目摘要

近年、大腸癌において発癌と腸内細菌叢(microbiome)の関連性が報告されるようになったが、これまでmicrobiomeとCMS分類との関連について検討した報告はない。特異的なmicrobiomeとCMS分類との相関が示せれば、遺伝子解析などを行わず簡便な便検体の菌叢解析のみで個別化治療選択や個別の予後予測が可能になるかもしれない。さらに、CMS分類と相関する特定のmicrobiomeを同定し、そのmicrobiomeのsubtype別の免疫微小環境の特徴をsingle cell RNA sequence (scRNA-seq) を用いて明らかにすることを目的とする。本年度は、大腸癌患者の術前の便検体を採取し、すでに100例以上の様々なサブタイプの大腸癌患者の便検体を得た。一部は菌叢解析に提出し、サブグループ別に比較し菌叢の違いを検索している。また、大腸癌10例以上、その他消化器癌を含めて100例以上の症例でscRNA-seqを行っており、免疫微小環境を構成する様々な免疫細胞やCAFのheterogeneity、各細胞亜集団の分化の流れや細胞間相互作用などについて解析をすすめている。CD4+ Tcell、CD8+ T cell、myeloid cell、fibroblastは、癌腫や発癌段階によって異なるheterogeneityを示し、これらの違いと菌叢の違いに相関があるかどうかを、今後検討していく。

In recent years, there has been an increase in the number of bacteria (microbiome) in cancer cells in recent years. In recent years, there have been many reports on the relationship between bacteria and bacteria (microbiome). In recent years, there have been many reports on bacteria in cancer, such as bacteria, bacteria and bacteria. The special microbiome CMS classifies each other to show you how to treat the bacteria in the stool, stool, stool The specific microbiome is the same as that of the specific microbiome, and the specific single cell RNA sequence (scRNA-seq) of the micro-environment is immunized to the specific single cell RNA sequence (scRNA-seq) according to the classification of specific single cell RNA sequence and scRNA-seq. In this year, there were more than 100 patients with major cancer who had stool before stool extraction. A book on the analysis of bacteria is proposed, and the results are compared to those of the bacteria. There were more than 10 cases of cancer and more than 10 cases of cancer of large intestine, and more than 100 cases of cancer of digestive organs of other organs. In the case of scRNA-seq, the patients were divided into two groups: CAF heterogeneity, cell aggregation, differentiation, cytosolic interaction, and cell cycle analysis. CD4+ Tcell, CD8+ Tcell, myeloid cell, fibroblast, cancer, cancer segment, tumor, heterogeneity, bacteria, bacteria, cancer, cancer