Adipose tissue stem cells and its extracellular vesicles could attenuate acute lung injury in a newborn porcine model of respiratory distress and ventilatory induced lung injury.

脂肪组织干细胞及其细胞外囊泡可以减轻新生猪呼吸窘迫和通气引起的肺损伤模型中的急性肺损伤。

• 批准号: 22K07874
• 负责人: Mohamed Hussein
• 金额: $ 2.66万
• 依托单位: Saitama Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K07874/
• 关键词: Preterm newborn; Chronic lung injury; Respiratory distress; Ventilatory induced; Lung injury; Adipose tissue; Stem cells; Extracellular vesicles; Ventilator Lung injury; Stem Cells; Newborn

Established a respiratory distress syndrome (RDS) model with ventilatory-induced lung injury (VILI) in a newborn piglet (1-2 days of life) showing pathophysiological changes seen in human preterm infants suffering from RDS.The RDS+VILI model shows a higher breathing rate, a higher oxygen needed to maintain an oxygen saturation above 90%, a higher partial carbon dioxide pressure, a higher oxygenation index, and a higher lung ultrasound score similar to the pathophysiological changes observed in human preterm infants suffering from RDS and could be used to evaluate new therapeutics for RDS. Repeated trials to collect adipose tissue and serum from a donor pig were done. Adipose tissue beads were cultured, and Adipose tissue driven stem cells were collected after 4-5 weeks.

建立新生仔猪呼吸窘迫综合征（RDS）合并呼吸机诱导肺损伤（VILI）模型RDS+VILI模型显示了较高的呼吸速率、维持氧饱和度高于90%所需的较高氧气、较高的二氧化碳分压、较高的氧合指数、较高的呼吸速率、较高的呼吸速率、较高的氧合指数、较高的呼吸速率、较高的呼吸速率（出生后1-2天），和较高的肺部超声评分，类似于在患有RDS的人类早产儿中观察到的病理生理学变化，并且可用于评估RDS的新疗法。进行重复试验以从供体猪收集脂肪组织和血清。培养脂肪组织珠，并在4-5周后收集脂肪组织驱动的干细胞。

项目成果

Adipose tissue stem cells and its extracellular vesicles could attenuate acute lung injury in a respiratory distress and ventilatory induced lung injury newborn porcine model.

脂肪组织干细胞及其细胞外囊泡可以减轻呼吸窘迫和通气诱导肺损伤新生猪模型中的急性肺损伤。

• 发表时间: 2023
• 期刊: Saitama Medical University Journal
• 作者: Mohamed Hamed Hussein;Fumihiko Namba;Takeshi Fujimoto;Haga Mitsuhiro;Marwa Saleh;Hisanori Sobajima;Kazuhiko Kabe;Koichi Moriwaki
• 通讯作者: Koichi Moriwaki