Adipose tissue stem cells and its extracellular vesicles could attenuate acute lung injury in a newborn porcine model of respiratory distress and ventilatory induced lung injury.

建立了一种新生猪(出生1-2天)呼吸窘迫综合征(RDS)与呼吸性肺损伤(VILI)的模型，该模型显示了人类早产儿RDS的病理生理变化。RDS+VILI模型显示出更高的呼吸频率，维持90%以上的氧饱和度所需的更高的氧气，更高的二氧化碳分压，更高的氧合指数，以及更高的肺超声评分，类似于人类早产儿RDS的病理生理变化，可用于评估RDS的新疗法。从供体猪身上收集脂肪组织和血清的重复试验已经完成。培养脂肪组织珠，4~5周后收集脂肪组织驱动干细胞。

Established a respiratory distress syndrome (RDS) model with ventilatory-induced lung injury (VILI) in a newborn piglet (1-2 days of life) showing pathophysiological changes seen in human preterm infants suffering from RDS.The RDS+VILI model shows a higher breathing rate, a higher oxygen needed to maintain an oxygen saturation above 90%, a higher partial carbon dioxide pressure, a higher oxygenation index, and a higher lung ultrasound score similar to the pathophysiological changes observed in human preterm infants suffering from RDS and could be used to evaluate new therapeutics for RDS. Repeated trials to collect adipose tissue and serum from a donor pig were done. Adipose tissue beads were cultured, and Adipose tissue driven stem cells were collected after 4-5 weeks.