Novel anti-fibrotic properties of heme binding proteins including Neuroglobin, Myoglobin, and Hemoglobin in comparison with Cytoglobin in vitro and in vivo

与细胞红蛋白相比，血红素结合蛋白（包括神经红蛋白、肌红蛋白和血红蛋白）在体外和体内的新型抗纤维化特性

• 批准号: 22K08083
• 负责人: LE THITHANHTHUY
• 金额: $ 2.75万
• 依托单位: Osaka Metropolitan University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K08083/
• 关键词: Globin; Cytoglobin; Neuroglobin; Myoglobin; Hemoglobin; Antioxidant; Anti-fibrosis

We have explored that other heme-binding proteins such as hemoglobin (HB), myoglobin (MB), and neuroglobin (NGB) which also possing the gas binding, and ROS scavenging function, have showed the same anti-fibrotic properties as CYGB. The antioxidant, and ROS scavenging function of human HB, MB, and NGB in comparison with CYGB as well as structure alteration regulating their function have been examined. Intra-cellular and tissue bio-distribution, the kinetics, safety assay, and anti-fibrotic function of these globins were demonstrated both in vitro and in vivo.

我们探索了其他与血红素结合的蛋白，如血红蛋白(Hb)、肌红蛋白(MB)和脑红蛋白(NGB)，它们也具有GAS结合和ROS清除功能，已经显示出与CyGB相同的抗纤维化特性。比较了人Hb、MB和NGB的抗氧化性和清除ROS的功能，以及调节其功能的结构变化。这些珠蛋白在细胞内和组织内的生物分布、动力学、安全性分析和抗纤维化功能在体外和体内都得到了证实。

项目成果

Capacity of extracellular globins to reduce liver fibrosis via scavenging reactive oxygen species and promoting MMP-1 secretion.

• DOI: 10.1016/j.redox.2022.102286
• 发表时间: 2022-06
• 期刊: Redox biology
• 影响因子: 11.4
• 作者: Hieu VN;Thuy LTT;Hai H;Dat NQ;Hoang DV;Hanh NV;Phuong DM;Hoang TH;Sawai H;Shiro Y;Sato-Matsubara M;Oikawa D;Tokunaga F;Yoshizato K;Kawada N
• 通讯作者: Kawada N

Cytoglobin, Neuroglobin, Myoglobin, but not Hemoglobin Exhibited Anti-Fibrotic Properties In Vitro and In Vivo

细胞红蛋白、神经球蛋白、肌红蛋白（但血红蛋白除外）在体外和体内表现出抗纤维化特性

• 发表时间: 2022
• 作者: Le Thi Thanh Thuy;Vu Ngoc Hieu;Hoang Hai;Dinh Viet Hoang;and Norifumi Kawada
• 通讯作者: and Norifumi Kawada

1.Extracellular globin therapy as a new strategy to combat fibrotic liver diseases

1.细胞外珠蛋白疗法作为对抗纤维化肝病的新策略

• 发表时间: 2022
• 作者: Thuy LTT (演者名);Hieu VN;Hai H;Kawada N
• 通讯作者: Kawada N

Cancer cells produce liver metastasis via gap formation in sinusoidal endothelial cells through proinflammatory paracrine mechanisms.

• DOI: 10.1126/sciadv.abo5525
• 发表时间: 2022-09-30
• 期刊: Science advances
• 影响因子: 13.6

1.Soluble immune checkpoint protein CD27 is a novel prognostic predictor of HCC development in HCV-SVR patients.

1.可溶性免疫检查点蛋白 CD27 是 HCV-SVR 患者 HCC 发展的新型预后预测因子。

• 发表时间: 2022
• 作者: Thuy LTT (演者名);Dong MP;Hai H;Hieu VN;Dinh VH;Uchida-Kobayashi S;Enomoto M;Ohtani N;Tamori A;Kawada N.
• 通讯作者: Kawada N.