頭頸部扁平上皮癌における転写因子p63/タイト結合分子JAM-Aの発現解析

转录因子p63/紧密连接分子JAM-A在头颈鳞癌中的表达分析

• 批准号: 22K09687
• 负责人: 黒瀬 誠
• 金额: $ 2.66万
• 依托单位: Sapporo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K09687/
• 关键词: 頭頸部癌; 免疫療法; タイトジャンクション; バイオマーカー

頭頸部扁平上皮癌におけるp63依存性JAM-Aを用いた補助診断への応用を目的としては、癌の悪性度におけるJAM-A/S100A7の発現および局在の変化を詳細に解析し、癌患者の血清中の可溶化JAM-Aを測定する。癌細胞および癌幹細胞に対しての分子標的治療への応用のために、p63依存性JAM-Aの発現調節機構を、癌細胞株、初代培養癌細胞、分離培養癌幹細胞および正常細胞を用いて総合的に解析する。前述を目的として、研究を継続しており、これまでのところ、頭頸部扁平上皮癌細胞において、p63,JAM-A,CLDN-1, HDAC1の高発現を見出した。また、JAM-AにおいてはmRNAレベルより増加が認められているを確認した。さらに癌患者血清において、可溶化JAM-Aの著しい増加も確認できた。さらに、JAM-Aを高発現している癌細胞株を用いたJAM-Aの発現調節機構を細胞内シグナルおよび糖代謝の面から解析しており、これまで、解析に適しているJAM-A高発現頭頸部扁平上皮癌株として、Detroit562細胞株を見出し、様々なシグナル伝達阻害剤を処置して解析を行っている。結果の一部として、JAM-Aは、癌の悪性化に密接な関与が考えられているWnt, EGFR, p38 MAPK, Hedgehogシグナルの関与が認められた。また癌細胞株に低酸素処置を行ったところ、p63およびJAM-Aの低下がみられ、JAM-AのsiRNA処置より癌細胞の増殖能、遊走能および浸潤能は明らかに低下していた。

Flat epithelial carcinoma of the part of the body. P63 dependent JAM-A was used to help cut off the tumor. The purpose of this study was to determine the value of soluble JAM-An in the serum of patients with cancer. The purpose of this study was to determine the degree of JAM-A/S100A7 in the serum of patients with cancer. For the treatment of cancer cells, tumor cells, tumor cells, p63-dependent JAM-A genes, cancer cell lines, primary culture cancer cells, isolated cancer cells, normal cells and normal cells, the combination of cell culture, p63-dependent DNA detection, primary culture, isolation and isolation of cancer cells were used. The aforementioned objectives, research methods, research methods, cell lines of flat epithelial carcinoma, p63 ~ (th) gamma, A ~ (nd) CLN ~ (1), and HDAC1 ~ (- 1) were studied. Please, JAM-A, please confirm that you have not received any confirmation from the mRNA. The serum of cancer patients was tested, soluble JAM-A was detected, and confirmation was added. The cell lines of cancer cell lines were detected by JAM-A, and the results were analyzed by JAM-An assay. The results showed that the cell lines of cancer cell lines were detected by JAM-An assay, and the results showed that the flat epithelial cell lines of cancer cell lines were detected by Elisa, Detroit562 cell lines were detected, and the results were detected by RT-PCR. Results the results showed that there was a close connection between Wnt, EGFR, p38 MAPK, Hedgehog gene and cancer gene. The cell line of cancer cell line was incubated with low acid concentration, p63 cell line, JAM-A cell line, p63 cell line, JAM-A cell line, JAM-A cell line, cell line and cell line.