Trilat. Project: Mango malformation: Epidemiology and simultaneous transcriptome analyses of host and fungal pathogen

三边形。

• 批准号: 63444630
• 负责人: Professor Dr. Günter Kahl (†)
• 金额: --
• 依托单位: Institut für Molekulare Biowissenschaften
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/63444630?language=en
• 关键词: Trilat.; Project; Mango; malformation; Epidemiology

Mango (Mangifera imtica) is an important fruit tree in Israel and has the potential of becoming a major crop in Palestine. One of the major bottlenecks for the development of this crop is a devastating disease - mango malformation - caused i by the fungus Fusarium mangiferae. Mango malformation is a complicated and quite mysterious disease, that causes malformation of the inflorescences, is spread throughout mango orchards in most parts of the world, and dramatically reduces yield, causing major losses to the formers. Neither a diagnostic tool nor a cure is currently available although they are badly needed. The specific goals of this proposal are as follows: 1. Simultaneous identification ofFvsarium and mango genes induced or repressed during the infection process, by SuperSAGE. 2. Epidemiological study of the source of the inoculum, the mechanisms of infection and the ways of dispersal of the pathogen. 3. Development of a molecular diagnostic tool for the detection of the pathogen in pre- symptomatic stages of the infection. The expected results of this project will provide novel insights into both the epidemiology and the molecular basis of the infection process leading to this unique fungal disease that are currently unavailable. Applying the novel SuperSage technique, we will gain in-depth insight into the transcriptomes of two intimately interacting organisms in vivo and the transcriptome controlling the formation of inflorescences in mango.

芒果（Mangifera imtica）是以色列的一种重要果树，并有可能成为巴勒斯坦的主要作物。这种作物发展的主要瓶颈之一是一种毁灭性的疾病-芒果畸形-由真菌芒果镰刀菌引起。芒果畸形病是一种复杂而神秘的病害，它导致花序畸形，在世界大部分地区的芒果园中广泛流行，严重影响芒果产量，给芒果园造成重大损失。目前既没有诊断工具，也没有治愈方法，尽管它们是迫切需要的。本建议的具体目标如下：1.利用SuperSAGE技术同时鉴定Fvsarium和mango在侵染过程中被诱导或抑制的基因。2.对接种物来源、感染机制和病原体传播方式的流行病学研究。3.开发用于在感染症状前阶段检测病原体的分子诊断工具。该项目的预期结果将为导致这种独特真菌疾病的感染过程的流行病学和分子基础提供新的见解。应用新的SuperSage技术，我们将深入了解体内两种密切相互作用的生物体的转录组以及控制芒果花序形成的转录组。