The foundness of colours and complex species taxonomy- a DNA based approach for revealing the genealogical structure in the European rose chafer (Cetonia aurata L.)

利用分子来解决分类学和种群结构的问题被认为是研究生物系统如何组织和推断其历史的最有前途的方法。替代传统的形态学为基础的分类和类型学分类，我们打算克服这种方法使用线粒体和核序列重建欧洲玫瑰金龟子Cetonia aurata，自然保护和最有吸引力的圣甲虫在中欧的问题闪光种的生物地理学的一些局限性。先前的初步序列分析揭示了线粒体DNA中一些不同的遗传结构，这些结构既不符合迄今为止定义的亚种，也不符合不同的地理分布。通过对该物种整个分布范围的不同种群的全面抽样，以及与之密切相关的物种，我们不仅将揭示该物种的历史地理学，包括其在冰川期的避难所，而且还试图探索目前的颜色多态性是否与该物种的遗传结构相关联。此外，我们将探讨如何到目前为止使用的形态charaters相关的树木模式获得。基于校准的树从更广泛的系统发育分析，我们计划引入一个时间尺度，我们的重建。这些结果将为我们提供有用的信息，在这个物种复杂的多样化模式和潜在的过程，导致物种的遗传结构。此外，我们希望在物种分类（物种划界）的关键和极端情况下，如在密切相关的类群，alloand parapatric种群和亚种的背景下，更详细地体验DNA分类方法。

Using molecules to address questions of taxonomy and population structure is perceived as the most promising approach to investigate how biological systems are organized and to infer about their history. Alternativly to traditional morphology-based taxonomy and typological classification, we intend to overcome some limitations of this approach using mitochiondrial and nuclear sequences to reconstruct the phylogeography of the European rose chafer Cetonia aurata, a flaship-species for issues of natural conservation and one of the most attractive scarab beetles in central Europe. Previous prelimiary sequence analyses have revealed some distinct genetic structure in mitochondrial DNA which is neither consistent with subspecies so far defined nor with different geographical occurrences. With a comprehensive sampling of the different populations of the entire distribution range of the species with respect to the numerous existing colour forms, and closely related species and we will uncover not only the historical biogeography of the species including their refugial areas during glaciation periods but also try to explore whether the present colour polymorphism is coupled in some exctend to the genetic structure in the species. In addition, we will explore how so far used morphological charaters correlate with the tree patterns obtained. Based on calibration of the tree from a wider phylogenetic analaysis we plan to introduce a time scale to our reconstructions. The results will provide us hopefully useful information about patterns of diversification in this species complex and the underlying processes leading to the genetic structure of the species. Furthermore, we hope to experience methodologies of DNA taxonomy more in detail in a context of critical and extreme cases of species taxonomy (species delimitation) such as in closely related taxa, alloand parapatric populations and subspecies.