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Study of Fertillization of the hagfish, Eptatretus burgeri (Agnatha)

盲鳗受精的研究

基本信息

批准号：
08833013
负责人：
MORISAWA Sachiko
金额：
$ 1.41万
依托单位：
St.Mariannna University, School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1998
项目状态：
已结题

项目摘要

Fertilization of the hagfish or myxiniformes, a member of the most primitive vertebrate group and and an animal of phylogenic interest, is unknown. I planned studies of the gametes with the aim of succeeding in fertilization of the species and obtained interesting results as described below.*Introduction of an acrosome reaction for spermatozoa in the hagfish, Eptatretus burgeri, was successfully achieved by treatment of mature spermatozoa with ionomycin and excess Ca2+. The spermatozoon produced an acrosomal process that elongated from the apex of the sperm head. The reaction bears resemblance to thet of invertebrate spermatozoa rather than that of vertebrate spermatozoa.*At the reaction, some vesicles were formed near the opening of the acrosomal vesicle. Such vesicles were observed at the exocytosis of the acrosomal vesicle in both Japanese and Pacific hagfish spermatozoa.*I investigated for the first time the fine structure of formation of the micropylar region in hagfish eggs during late stages of oogenesis, focusing on the bottom region of the micropyle and the egg surface. During these stages many micropylar cells penetrated through the chorion and reached a pit of the egg surface. The cells were separated from the chorion by a wall of amorphous material and from the egg surface by another fibrous layer in the pit. In the later stages, the stack of the micropylar cells loosoned, and a space appeared between the stack and the layer on the egg surface. The structures are markedly different from those observed in the same region of teleost fishes. A hypothesis that a possible relationship between egg and sperm morphology from protochordates to teleosts was suggested.*We investigated that proteasomes regulate the motility of salmonid fish sperm through modulation of cAMP-dependent phosphorylation of an outer arm dynein light chain.

鳗鱼或粘鱼是最原始的脊椎动物群体中的一员，也是一种具有系统发育意义的动物，其受精情况尚不清楚。我计划对配子进行研究，目的是成功地使该物种受精，并获得了如下所述的有趣结果。*通过用离子霉素和过量的钙离子处理成熟精子，成功地将顶体反应引入到八角鳗精子中。精子产生一个顶体突起，从精子头部的顶端伸长。这种反应类似于无脊椎动物精子，而不是脊椎动物精子。在反应过程中，在顶体小泡开口附近形成了一些小泡。在日本和太平洋鳗鱼精子的顶体囊泡的胞吐过程中都观察到了这样的小泡。*我首次研究了鳗鱼卵子在卵子发生后期形成微孔区的精细结构，重点是珠孔的底部和卵的表面。在这些阶段，许多珠孔细胞穿过绒毛膜，到达卵子表面的一个凹坑。细胞与绒毛膜被一层无定形物质隔开，与卵子表面隔开的是凹陷中的另一层纤维层。后期，珠孔细胞堆积松动，与卵子表面的层之间出现间隙。这些结构与在同一区域观察到的硬骨鱼的结构明显不同。提出了一个假设，即从原脊索动物到硬骨鱼的卵子和精子形态之间可能存在关系。*我们研究了蛋白酶体通过调节依赖于cAMP的外臂动力蛋白轻链的磷酸化来调节鲑鱼精子的活力。