Identification and functional characterization of proteins associated with membrane fractions surrounding activating and inhibitory Natural Killer cell receptors

围绕激活和抑制自然杀伤细胞受体的膜组分相关蛋白的鉴定和功能表征

• 批准号: 68359804
• 负责人: Professor Dr. Carsten Watzl
• 金额: --
• 依托单位: Leibniz-Institut für Arbeitsforschung an der TU Dortmund (IfADo)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2008-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/68359804?language=en
• 关键词: Identification; functional; characterization; proteins; associated

Natural Killer (NK) cells play an important role in the immune response against viral infections and cancer. The function of NK cells is regulated by a balance of positive and negative signals transmitted by activating or inhibitory cell surface receptors. The membrane domains surrounding these receptors play an integral role in the regulation of NK cell activity. While activating receptors need to associate with specialized membrane domains (Detergent resistant membrane fractions and GM1- rich microdomains) in order to activate NK cell functions, inhibitory receptors prevent the association of activating receptors with these domains and thereby control NK cell activity. We will therefore study the distribution of membrane microdomains during NK cell activation and inhibition. By identification and functional characterization of specific protein components of membrane domains surrounding activating and inhibitory receptors in NK cells we aim to get a better understanding of NK cell regulation and to identify novel targets for the manipulation of NK cell activity.

自然杀伤（NK）细胞在针对病毒感染和癌症的免疫应答中发挥重要作用。NK细胞的功能受通过激活或抑制细胞表面受体传递的正信号和负信号的平衡调节。这些受体周围的膜结构域在NK细胞活性的调节中起着不可或缺的作用。虽然活化受体需要与专门的膜结构域（抗洗涤剂膜部分和富含GM1的微结构域）结合以活化NK细胞功能，但抑制性受体阻止活化受体与这些结构域结合，从而控制NK细胞活性。因此，我们将研究NK细胞活化和抑制过程中膜微区的分布。通过对NK细胞中激活和抑制受体周围膜结构域的特异性蛋白质组分的鉴定和功能表征，我们的目标是更好地了解NK细胞的调节，并确定操纵NK细胞活性的新靶点。