Functions of the major membrane microdomain protein reggie/flotillin in formation of highly mobile forms of Wnt and Hedgehog morphogens in Drosophila

主要膜微结构域蛋白 reggie/flotillin 在果蝇中形成高度移动形式的 Wnt 和 Hedgehog 形态发生素中的功能

• 批准号: 74893249
• 负责人: Professor Dr. Vladimir L. Katanaev, Ph.D.
• 金额: --
• 依托单位: Department of Cell Physiology and Metabolism
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/74893249?language=en
• 关键词: Functions; major; membrane; microdomain; protein

The lipid-modified morphogens Wnt and Hedgehog are secreted and spread through the tissue by at least two pathways. The first results in formation of a poorly mobile morphogen pool, and activates short-range target genes. The second forms a more mobile morphogen pool required for activation of longrange targets. We have demonstrated that this second secretion route depends in Drosophila on the function of one of the major membrane microdomain organizing proteins, reggie-1/flotillin-2. The aims of the current project are: a) to understand the molecular nature of the reggie-dependent, highly mobile form of the Wnt and Hh morphogens; b) to investigate the morphogen secretion routes and their dependence on reggie-1 at the ultrastructural level; and c) to elucidate involvement of reggie-1 in other signaling pathways during Drosophila wing development.

脂质修饰的形态发生素Wnt和Hedgehog通过至少两种途径分泌并通过组织扩散。第一种导致形成移动的较差的形态原库，并激活短程靶基因。第二种形成了一个更移动的形态素库，需要激活的远程目标。我们已经证明，这第二个分泌途径依赖于在果蝇的主要膜微区组织蛋白，reggie-1/flotillin-2的功能。本项目的目的是：a）了解reggie依赖的，高度移动的形式的Wnt和Hh形态发生剂的分子性质; B）研究形态发生剂的分泌途径及其在超微结构水平上对reggie-1的依赖性;和c）阐明reggie-1在果蝇翅膀发育过程中参与的其他信号通路。