液体架橋を用いた局所環境制御による光合成細胞評価技術の開拓

开发利用液体交联的局部环境控制的光合细胞评估技术

• 批准号: 22KJ0942
• 负责人: 金子 真悟
• 金额: $ 1.09万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2023
• 资助国家: 日本
• 起止时间: 2023-03-08 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22KJ0942/
• 关键词: ドロップレットマイクロ流体力学; 画像認識; 藍藻; MEMS

令和4年度は　高速浸透圧刺激を藍藻に与えた際の応答を単一細胞レベルで解析する技術開拓に取り組み，それぞれ国内学会，国外学会で一件ずつ発表した．国内学会では，“日本機械学会 ロボティクス・メカトロニクス講演会，「急激な浸透圧変化に対する藍藻の浸透圧 調製 機能の評価」のタイトルで発表し，高速浸透圧刺激を与えた際の単一細胞解析を実施し，細胞の応答に不均一性が観察されることを確認した．国際学会は，International Symposium on Micro/nano mechatoronics and Human Science (MHS)において，“Evaluation of viability of single synechocystis sp. pcc 6803 in reaction to high-speed osmotic pressure change,” というタイトルで発表を行った．この発表では，高速浸透圧刺激を与えた際の応答の不均一性が細胞膜の透過率の違いに起因することを解明しその内容を発表した．

In 2004 and 2005, the high-speed penetration pressure stimulation of cyanobacteria and the interaction between them were analyzed by a single cell. The technology development group was selected from domestic and foreign institutes. The National Society of Machinery presented a lecture entitled "Evaluation of the Impregnation Pressure Modulation Function of Cyanobacteria in Response to Impregnation Pressure Stimulation", which was held at the Japanese Society of Machinery. The analysis of single cells during high speed impregnation pressure stimulation was carried out and the heterogeneity of cell response was observed. International Symposium on Micro/Nano Mechanics and Human Science (MHS),"Evaluation of viability of single synechocystis sp. pcc 6803 in reaction to high-speed osseous pressure change," International Symposium on Micro/Nano Mechanics and Human Science (MHS),"Evaluation of viability of single synechocystis sp. pcc 6803 in reaction to high-speed osseous pressure change." The results show that the nonuniformity of the response between the high speed osmotic pressure stimulation and the high speed osmotic pressure stimulation is the cause of the nonuniformity of the cell membrane permeability.

项目成果

Evaluation of viability of single synechocystis sp. pcc 6803 in reaction to high-speed osmotic pressure change

单个集胞藻的生存能力评估。

• 发表时间: 2022
• 作者: Shingo Kaneko;Masaru Tsujii;Nobuyuki Uozumi;Fumihito Arai
• 通讯作者: Fumihito Arai

急激な浸透圧変化に対する藍藻の浸透圧 調製 機能の評価

蓝绿藻响应渗透压快速变化的渗透压调节功能评价

• 发表时间: 2022
• 作者: Y. Shun;T. Kusumoto;Y. Sugawara;O. Mori;楠本哲也，森治;楠本哲也，保田瞬，菅原佳城，森治;保田瞬，楠本哲也，菅原佳城，森治;金子真悟
• 通讯作者: 金子真悟