BIOLOGICAL MEANING OF MAJOR CYTOKINININ COCONUT MILK.

一种从椰子胚乳中分离得到的细胞分裂素。（椰奶），占总细胞分裂素活性的20%以上，通过NMR技术、质谱和通过高效液相色谱（HPLC）的糖分析进行结构分析。从核磁共振和质谱数据推断出细胞分裂素的平面结构。糖部分的结构，包括其绝对结构，通过HPLC分析源自细胞分裂素的糖醇乙酸酯和糖腈乙酸酯来确定。通过NMR确定糖-糖键的构型，并最终确定其结构为14-O-{3-O-[β-D-半乳糖基-（1 *2）-α-D-半乳糖基-（1*3）-α-L-阿拉伯呋喃糖基]-4-O-（α-L-阿拉伯呋喃糖基）-β-D-吡喃半乳糖基}-反式玉米素核苷（GAZER）。用N-苯基-N' -（4-吡啶基）脲（4PU）-Sepharose 4 B亲和凝胶对绿豆（Vigna radiata）进行了CSBP的分离纯化，测定了CSBP的部分氨基酸序列，并通过逆转录PCR（RT PCR）技术获得了CSBP的cDNA，该cDNA编码的蛋白分子量为17 kDa。基于数据的同源性搜索显示，CSBP在一个主要的花粉过敏原/发病相关蛋白家族的新成员。

A cytokinin isolated from the fluid endosperm of Cocos mucifera L.(coconut milk), accounting for more than 20% of the total cytokinin activity, was structurally analyzed by NMR techniques, mass spectrometry, and sugar analysis by high performance liquid chromatography (HPLC). The planar structure of the cytokinin was deduced from its NMR and mass spectrometric data. The structure of the sugar moiety, including its absolute structure, was determined by HPLC analysis of alditol acetates and aldononitrile acetates derived from the cytokinin. The configuration of the sugar-sugar bonds was determined by NMR, and the structure was finally identified as 14-O-{3-O-[beta-D-galactopyranosyl-(l*2)-alpha-D-galactopyranosyl-(1*3)-alpha-L-arabinoffuranosyl]-4-O-(alpha-L-arabinofuranosyl)-beta-D-galactopyranosyl}-trans-zeatin riboside (GAZER).Cytokinin-specific binding protein (CSBP) was purified from Mung Bean (Vigna radiata) by the use of N-phenyl-N' -(4-pyridyl)urea (4PU)-Sepharose 4B, an affinity gel liganded with 4PU.We determined partial amino acid sequences of CSBP and isolated its cDNA by reverse-transcription (RT) PCR.The cDNA encoded a protein with a calculated molecular mass of 17 kDa. A data based homology search revealed that CSBP in a novel member of a major pollen allergen/pathogenesis-related protein family.