Analysis of the Rho small GTP-binding proteinmediated signaling cascade

Rho 小 GTP 结合蛋白介导的信号级联分析

• 批准号: 09680693
• 负责人: TANAKA Kazuma
• 金额: $ 2.11万
• 依托单位: HOKKAIDO UNIVERSITY (1998)Osaka University (1997)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09680693/
• 关键词: Small GTP-binding protein; Cascade; Budding yeast; Cell polarity; Rho; Actin cytoskeleton; BNI1; BNR1

The small GTP-binding proteins, including Rho, are implicated in the signaling cascade which regulates actin cytoskeleton-dependent cell functions. In this study, we have studied this small GTP-binding protein cascade at a molecular level.Regulation of activation of PHO1 : We have isolated and analyzed proteins which interact with ROM1/ROM2, GDPIGTP exchange factors for RHO1.Modes of action of RHO1 : We have isolated new proteins, SPA2 and SHS1, which bind BNI1, a potential target for PHO1.SPA2 is required for the localization of RHO1, and SHS1 is involved in cyotokinesis.Modes of action of BNR1 : We have isolated an SH3 domain-containing HOF1, as a protein which bind BNI1 related protein, BNR1. Both HOF1 and BNP1 are localized at the cell division site and involved in cytokinesis. As described above, we have analyzed PHO1-mediated small GTP-binding protein cascade and obtained new findings in the cascade at a molecular level.

小的GTP结合蛋白，包括Rho，参与了调节肌动蛋白细胞骨架依赖的细胞功能的信号级联反应。在这项研究中，我们在分子水平上研究了这个小的GTP结合蛋白级联。PHO1的激活调控：我们分离并分析了与ROM1/ROM2，GDPIGTP交换因子相互作用的蛋白质RHO1。RHO1的作用模式：我们分离到了与BNI1结合的新蛋白SPA2和SHS1，BNI1是PHO1的潜在靶标。SPA2是RHO1定位所必需的，SHS1参与细胞分裂。HOF1和BNP1均定位于细胞分裂部位，参与胞质分裂。如上所述，我们已经分析了PHO1介导的小GTP结合蛋白级联，并在分子水平上获得了新的发现。

项目成果

Tanaka,K.: "Control of reorganization of the actin cytoskeleton by Rho family small GTP-binding proteins in yeast." Curr.Opin.Cell Biol.10・1. 112-116 (1998)

Tanaka, K.：“酵母中 Rho 家族小 GTP 结合蛋白对肌动蛋白细胞骨架重组的控制。”Curr.Opin.Cell Biol.10·1 (1998)。

Kamei.T..et al.: "Interaction of Bnr1p with a novel Src homology 3 domain-containing Hof1p" J.Biol.Chem.273. 28341-28345 (1998)

Kamei.T..等人：“Bnr1p 与包含新 Src 同源 3 结构域的 Hof1p 的相互作用”J.Biol.Chem.273。

Fujiwara,T.: "Rho1p-Bni1p-Spa2p interactions - Implication in localization of Bni1p at the bud site and regulation of the actin cytoskeleton in Saccharomyces cerevisiae." Mol.Biol.Cell. (in press). (1998)

Fujiwara,T.：“Rho1p-Bni1p-Spa2p 相互作用 - 对酿酒酵母中 Bni1p 芽位点定位和肌动蛋白细胞骨架调节的影响。”

Koch, G.: "Association of the Rho family small GTP-binding proteins with Rho GDP dissociation inhibitor(Rho GDI)in Saccharomyces cerevisiae" Oncogene. 15. 417-422 (1997)

Koch, G.：“酿酒酵母中 Rho 家族小 GTP 结合蛋白与 Rho GDP 解离抑制剂 (Rho GDI) 的关联”致癌基因。

Kamei, T.: "Interaction of Bnr1p with a novel Src homology 3 domain-containing Hof1p" J.Biol.Chem.273・43. 28341-28345 (1998)

Kamei, T.：“Bnr1p 与包含新 Src 同源 3 结构域的 Hof1p 的相互作用”J.Biol.Chem.273·43 28341-28345 (1998)。