Molecular genetic study on mechanisms of auxin action controlling growth of higher plants

生长素控制高等植物生长机制的分子遗传学研究

• 批准号: 09680717
• 负责人: YAMAMOTO Kotaro
• 金额: $ 2.11万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09680717/
• 关键词: Auxin; Auxin-resistant mutant; Arabidopsis thaliana; Growth curvature; Gravitropism; Phototropism; Epinasty; Auxin influx carrier; 上備成長; 成長; 輸送担体; 突然変異体

We isolated an Arabidopsis mutant, msg1, which was defective in growth curvature response of hypocotyl induced by unilateral application of auxin. Msg1 was found allelic to the nonphototropic mutant, nph4 (Liscum and Briggs, 1995). msg1 hypocotyls were impaired in gravitropism as well as the auxin-induced growth curvature and phototropism ; they were resistant to auxin. msg1 was similar to wild type in shape except for epinastic or hyponastic rosette leaves. These results indicate that msg1 is defective in auxin-specific growth curvature responses. We checked many growth mutants of Arabidopsis with respect to the auxin-induced growth curvature response. But, we found that only axr1 lacked the growth response. Axr1 hypocotyls were impaired in both gravitropism and phototropism, indicating that axr1 was another auxin-specific growth curvature mutant. in order to determine the relationship between MSG1 and AXR1 in the auxin-dependent growth curvature response, we constructed a double muta … More nt between msg1 and axr1, and measured its growth curvature responses. Both msg1 and axr1 single mutants lost phototropism of hypocotyl partially ; msg1 axr1 double mutants displayed no phototropism of hypocotyl. Gravitropism of both single mutants was affected partially ; defects in hypocotyl gravitropism of the double mutant were larger than those of either single mutant. Axr1 rosette leaves were wrinkled, while msg1 leaves were epinastic ; leaves of msg1 axr1 double mutant were wrinkled and epinastic. These results indicate that effects of mutations in msg1 and axr1 were additive. In other words, we can conclude that MSG1 and AXR1 act independently in auxin-specific growth curvature responses.We also investigated effects of indole-3-acetic acid, 2,4-dichhlorophenoxyacetic acid and 1-naphthaleneacetic acid on abberant gravitropism of aux1 roots. It was found that only an addition of naphthaleneacetic acid restored aux1 gravitropism. This finding as well as the predicted amino acid sequence of AUX1 protein Q3ennett et al., 1996) indicates that A UX1 probably encodes an auxin influx carrier. Less

我们分离到一个拟南芥突变体msg 1，该突变体在单方面施加生长素诱导下胚轴生长弯曲反应中存在缺陷。发现Msg 1与非趋光突变体nph 4是等位基因（Liscum和Briggs，1995年）。msg 1下胚轴的向重力性以及生长素诱导的生长弯曲和向光性受到损害;它们对生长素具有抗性。msg 1除了上生或下生莲座丛叶外，在形状上与野生型相似。这些结果表明msg 1在生长素特异性生长曲率反应中是有缺陷的。我们检查了许多生长突变体的拟南芥在生长素诱导的生长曲率反应。但是，我们发现只有axr 1缺乏生长反应。Axr 1下胚轴的向重力性和向光性都受到了损害，表明axr 1是另一个生长素特异性生长弯曲突变体。为了确定MSG 1和AXR 1在生长素依赖的生长曲率响应中的关系，我们构建了一个双穆塔， ...更多信息 nt之间，并测量其生长曲率反应。msg 1和axr 1单突变体均部分丧失了下胚轴的向光性，msg 1 axr 1双突变体不显示下胚轴的向光性。这两个单突变体的向重力性的影响部分，在下胚轴向重力性的双突变体的缺陷是大于任何单突变体。axr 1莲座叶起皱，msg 1叶片上位，msg 1 axr 1双突变体叶片起皱且上位。这些结果表明msg 1和axr 1的突变效应是加性的。我们还研究了吲哚-3-乙酸、2，4-二氯苯氧乙酸和1-萘乙酸对aux 1根异常向重力性的影响。结果发现，只有另外的萘乙酸恢复aux 1向重力性。这一发现以及预测的AUX 1蛋白质的氨基酸序列Q3 ennett等人，1996）表明，A UX 1可能编码生长素流入载体。少

项目成果

Watahiki, M.K., Tatematsu, K., Fujihira, K., Yamamoto, M.and Yamamoto, K.T.: "The MSG1 and AXR1 genes of Arabidopsis are likely to act independently in growth-curvature responses of hypocotyl" Planta. 207. 362-369 (1999)

Watahiki, M.K.、Tatematsu, K.、Fujihira, K.、Yamamoto, M. 和 Yamamoto, K.T.：“拟南芥的 MSG1 和 AXR1 基因可能在下胚轴的生长曲率反应中独立发挥作用” Planta。

M.Yamamoto & K.T.Yamamoto: "Differeutial cffects of 1-naphthaleneacetic acid indde-3-autic acid and 2,4-dichlaropheroxyacetic acid on the gravitvopic……" Plant and Cell Physiology. 39. 660-664 (1998)

M. Yamamoto 和 K. T. Yamamoto：“1-萘乙酸 indde-3-autic 酸和 2,4-二氯过氧乙酸对重力的影响……”植物和细胞生理学 39. 660-664 (1998)。

M.K.Watahiki & K.T.Yamamoto: "The massugu1 mutation of Arabidopsis identitied with failure of auxiu-induced growth curratuoe of hypocotyl conters auxin inseusitivity……" Plaut Physiology. 115. 419-426 (1997)

M.K.Watahiki 和 K.T.Yamamoto：“拟南芥的 Massugu1 突变被鉴定为下胚轴辅助诱导的生长失败导致生长素不连续性......”Plaut Physiology 115. 419-426 (1997)。

M.Yamamoto: "Differential effects of 1-naphthaleneacetic acid,indole-3-acetic acid and 2,4-dichlorophenoxyacetic acid on the gravitropic response of roots…" Plant and Cell Physiology. 39(印刷中). (1998)

M. Yamamoto：“1-萘乙酸、吲哚-3-乙酸和 2,4-二氯苯氧基乙酸对根向重力反应的不同影响……”植物和细胞生理学 39（出版中）。

M.K.Watahiki & K.T.Yamamoto: "The massugu1 mutation of Arabidopsis identified with failure of auxin-induced growth curvature of hypocotyl・・・" Plant Physiology. 115. 419-426 (1997)

M.K.Watahiki 和 K.T.Yamamoto：“拟南芥的 Massugu1 突变导致生长素诱导的下胚轴生长曲率失败......”植物生理学 115. 419-426 (1997)。