The center of biological rhythm and neuroendocrine system

生物节律和神经内分泌系统的中枢

• 批准号: 09680797
• 负责人: TAMADA Yoshitaka
• 金额: $ 2.11万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09680797/
• 关键词: suprachiasmatic nucleus; ptotic stimulation; hypothalamus; circadian rhythm; astrocyte; optic nerve; サーカデイアンリズム; 生体リズム; 神経内分泌; Immediate early gene; VIPニューロン; 一酸化窒素

(1) Photic induction of NGFI-A gene expression was investigated in the SCN using in situ hybridization histochemistry. The broadness of NGFI-A mRNA and c-fos mRNA expression after photic stimulation were different. And NGFI-A gene induced in these cells of the SCN including VIP neurons are considered to be involved in circadian entrainment by light (Brain Res., 1997). (2) According to aging, the expression of VIP mRNA in the SCN was markedly decreased and their rhythm was disappeared (Neurosci.Lett., 1997). (3) The retinal projections to gastrin-releasing peptide (GRP) neurons in the SCN were investigated. The photic information is considered to be directly input from the optic nerve to GRP neurons and GRP neurons may be involved in circadian entrainment by light (NeuroReport 1997). (4) To explore the effect of photic stimulation in the astroglial elements in the hypothalamus, GFAP-like immunoreactive elements in the SCN and median eminence was investigated morphologically (Brain Res.Bull, 1998 ; Acta Histocem.Cytochem., 1998).

(1)采用原位杂交组织化学方法研究了SCN中NGFI-A基因表达的光诱导。光刺激后NGFI-A mRNA和c-fos mRNA表达的广度不同。并且在SCN的这些细胞（包括VIP神经元）中诱导的NGFI-A基因被认为参与光的昼夜节律夹带（Brain Res.，1997年）。(2)随着年龄的增长，SCN中VIP mRNA的表达显著降低，并且它们的节律消失（Neurosci.Lett.，1997年）。(3)观察视网膜对SCN内胃泌素释放肽（GRP）神经元的投射。光信息被认为是从视神经直接输入到GRP神经元，并且GRP神经元可能参与光的昼夜节律夹带（NeuroReport 1997）。(4)为了探索光刺激对下丘脑中星形胶质细胞成分的影响，从形态学上研究了SCN和正中隆起中的GFAP样免疫反应成分（Brain Res.Bull，1998 ; Acta Histocem. Cytochem.，1998年）。

项目成果

Masaki Tanaka: "Direct retinal projections to GRP neurons in the suprachiasmatic nucleus of the rat." Neuro Report. 8. 2187-2191 (1997)

Masaki Tanaka：“直接视网膜投射到大鼠视交叉上核中的 GRP 神经元。”

Tamada, Y., et al.: "Neuron-glia Interaction in the suprachicsmatic Nuclens-A double labeling light and electron Microscoptic immunocytochemical study in the rat." Brair Res.Bull.45. 281-287 (1998)

Tamada, Y., et al.：“大鼠上性 Nuclens 中的神经元-胶质细胞相互作用——双标记光和电子显微镜免疫细胞化学研究”。

Masaki Tanaka: "Nitrergic neurons in the medial amygdala project to the hypothalamic paraventricular nucleus of the rat." Brain Research. 777. 13-21 (1997)

Masaki Tanaka：“内侧杏仁核中的氮能神经元投射到大鼠的下丘脑室旁核。”

Tanaka, M., AmayaF., Tamada, Y., Okamura, H., Hisa, Y.and Ibata, Y.: "Induction of NGFI-A gene expression in the rat suprachiasmatic nucleus by photic stimulation" Brain Res.756. 305-310 (1997)

Tanaka, M.、AmayaF.、Tamada, Y.、Okamura, H.、Hisa, Y. 和 Ibata, Y.：“光刺激诱导大鼠视交叉上核中 NGFI-A 基因表达”Brain Res.756。

Tamada Y. et al: "Morphological interections between glial fibrillary actidicprotein (GFAP)-like immunoreactive elements and latenizing hormone ufeseing hnmone (LHRH)-like immunoreactive nerve endirgs in the median eminexis of the rat-Donble laloreling im

Tamada Y. 等人：“大鼠正中阴道中神经胶质原纤维活性蛋白 (GFAP) 样免疫反应元件和乳化激素 ufeseing hnmone (LHRH) 样免疫反应神经末端之间的形态学相互作用-Donble laloreling im