Establishment of the osteopetrosis model in mice

小鼠FRP-1(MFRP-1)和Ly10抗原是同种异体抗原。Ant-Ly10.1单抗能与稳定表达MFRP-1.1的HeLa细胞(HeLa Cells/MFRP-1.1)反应，但不与HeLa细胞反应！另一方面，抗Ly10.2单抗能与HeLa/MFRP-1.2细胞反应，但不与HeLa细胞[S/MFRP-1.1]反应。这些发现表明，Ly10.1和Ly10.2抗原分别与MFRP-1.1和rnFRP-1.2分子的重链相同。CDF1和BDF1小鼠的胸腺细胞和成纤维细胞对抗Ly10.1和抗Ly10.2/FRP-2抗体均有反应，表明MFRP-I/Ly10在F1小鼠体内有共表达。令人惊讶的是，来自CDFL小鼠的DBT细胞由两个不同的细胞群组成：一个同时表达MFRP-1.1和MFRP-1.2抗原，另一个仅表达MFRP-1.1抗原。因此，我们试图通过限制稀释法来分离克隆的DBT细胞系。最后获得了6个克隆的DBT细胞系，其中3个克隆的DBT细胞同时表达MFRP-1.1和MFRP-1.2，另外3个克隆的DBT细胞同时表达MFRP-1.1和MFRP-1.2，包括克隆5的克隆细胞只表达MFRP-L1抗原。在DBT克隆5细胞和IMC细胞中均未检测到nYFRP-1.2mRNA和编码MFRP-1.2的基因组克隆，提示CDF1小鼠来源细胞中MFRP-L/Lylo异型基因的异常表达是基因缺失所致。FRP-1缺陷小鼠的项目正在进行中。

Murine FRP-1 (mFRP-1) and Ly10 antigens are alloantigens. Antl-Ly10.1, monoclonal antibody (mAb) reacts to HeLa cells stably expressing mFRP-1.1 (HeLa cells/mFRP-1.1), but does not react to HeLa cells! rnFRP-1.2, On the other hand, anti-Ly10.2 mAb reacts to HeLa/mFRP-1.2 cells, but does not react to HeLa cel]s/mFRP-1.1. These findings indicate that Ly10.1 and Ly10.2 antigens are identical to the heavy chains of mFRP-1.1 and rnFRP-1.2 molecules, respectively. Thymocytes and fibroblast cells obtained from CDF1 and BDFl mice showed reactivity to both anti-Ly10.1 and anti-Ly10.2/FRP-2 antibodies, indicating that mFRP-I /Ly1 0 alloantigens are codominantly expressed In the Fl mice. Surprisingly, DBT cells, derived from CDFl mice, consist of two different ceilpopulations : one expresses both mFRP-1.1 and mFRP-1.2 antigens and the other expresses only mFRP-1.1 antigen. Consequently, we tried to isolate cloned DBT cell lines by limiting dilution. Finally, six cloned DBT cell lines were obtained, and three clones of these cloned DBT cells expressed both mFRP-1.l and mFRP-1.2, and another three clones of these cloned DBT cells expressed both mFRP-1.1 and mFRP-1.2, and another three clones including clone 5 cells expressed only mFRP-L1 antigen. Neither nYFRP-1.2 mRNA nor the genomic cDNA clone encoding mFRP-1.2 could be detected in DBT clone 5 cells, nor could the genomic cDNA encoding rnFRP-1.1 be detected in IMC cells, indicating that aberrant expression of mFRP-l/LylO allotypes in CDF1 mice-derived cells is due to the gene deletlon.. The project of FRP-1 dificient mice is in progress.