Establishment of the osteopetrosis model in mice

小鼠石骨症模型的建立

• 批准号: 09680830
• 负责人: TSUMURA Hideki
• 金额: $ 1.86万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09680830/
• 关键词: Osteoclast; FRP; Monoclonal antibody; 骨粗鬆症; 細胞融合

Murine FRP-1 (mFRP-1) and Ly10 antigens are alloantigens. Antl-Ly10.1, monoclonal antibody (mAb) reacts to HeLa cells stably expressing mFRP-1.1 (HeLa cells/mFRP-1.1), but does not react to HeLa cells! rnFRP-1.2, On the other hand, anti-Ly10.2 mAb reacts to HeLa/mFRP-1.2 cells, but does not react to HeLa cel]s/mFRP-1.1. These findings indicate that Ly10.1 and Ly10.2 antigens are identical to the heavy chains of mFRP-1.1 and rnFRP-1.2 molecules, respectively. Thymocytes and fibroblast cells obtained from CDF1 and BDFl mice showed reactivity to both anti-Ly10.1 and anti-Ly10.2/FRP-2 antibodies, indicating that mFRP-I /Ly1 0 alloantigens are codominantly expressed In the Fl mice. Surprisingly, DBT cells, derived from CDFl mice, consist of two different ceilpopulations : one expresses both mFRP-1.1 and mFRP-1.2 antigens and the other expresses only mFRP-1.1 antigen. Consequently, we tried to isolate cloned DBT cell lines by limiting dilution. Finally, six cloned DBT cell lines were obtained, and three clones of these cloned DBT cells expressed both mFRP-1.l and mFRP-1.2, and another three clones of these cloned DBT cells expressed both mFRP-1.1 and mFRP-1.2, and another three clones including clone 5 cells expressed only mFRP-L1 antigen. Neither nYFRP-1.2 mRNA nor the genomic cDNA clone encoding mFRP-1.2 could be detected in DBT clone 5 cells, nor could the genomic cDNA encoding rnFRP-1.1 be detected in IMC cells, indicating that aberrant expression of mFRP-l/LylO allotypes in CDF1 mice-derived cells is due to the gene deletlon.. The project of FRP-1 dificient mice is in progress.

鼠FRP-1（mFRP-1）和Ly 10抗原是同种异体抗原。Antl-Ly 10.1，单克隆抗体（mAb）与稳定表达mFRP-1.1的HeLa细胞（HeLa细胞/mFRP-1.1）反应，但不与HeLa细胞反应！另一方面，抗Ly10.2 mAb与HeLa/mFRP-1.2细胞反应，但不与HeLa细胞/mFRP-1.1反应。这些发现表明Ly10.1和Ly10.2抗原分别与mFRP-1.1和rnFRP-1.2分子的重链相同。从CDF 1和BDF 1小鼠获得的胸腺细胞和成纤维细胞显示出对抗Ly10.1和抗Ly10.2/FRP-2抗体的反应性，表明mFRP-I /Ly 10同种异体抗原在F1小鼠中共显性表达。令人惊讶的是，来源于CDF 1小鼠的DBT细胞由两种不同的细胞群组成：一种表达mFRP-1.1和mFRP-1.2抗原，另一种仅表达mFRP-1.1抗原。因此，我们尝试通过有限稀释法分离克隆DBT细胞系。最后，获得6个克隆DBT细胞系，这些克隆DBT细胞中的3个克隆同时表达mFRP-1.1和mFRP-1.2，另外3个克隆同时表达mFRP-1.1和mFRP-1.2，另外3个克隆包括克隆5细胞仅表达mFRP-L1抗原。在DBT克隆5细胞中既不能检测到nYFRP-1.2 mRNA，也不能检测到编码mFRP-1.2的基因组cDNA克隆，在IMC细胞中也不能检测到编码rnFRP-1.1的基因组cDNA，表明mFRP-1/Lyl 0同种异型在CDF 1小鼠来源的细胞中的异常表达是由于基因缺失。FRP-1缺陷小鼠的研究正在进行中。

项目成果

Tsumura H, et al.: "Isolation and characterization of monoclonal antibodies directed against murine FRP-1/CD98/4F2 heavy chain" Immunology and Cell Biology. 77. 19-27 (1999)

Tsumura H 等人：“针对鼠 FRP-1/CD98/4F2 重链的单克隆抗体的分离和表征”免疫学和细胞生物学。

Tsumura H, et al: "Mouse Alloantigen Lyic is identical to Murine Fusion Regulatory Protein/4F2/CD98" Cellular Immunology. 184. 153-160 (1998)

Tsumura H 等人：“小鼠同种异体抗原 Lyic 与鼠融合调节蛋白/4F2/CD98 相同”细胞免疫学。

Tsumura H,et al.: "Isolation and characterization of monodonal autibodies divected against murine FRP-1/CD98/4F2 heavy chain" Immunology and Cell Biology. 77. 19-27 (1999)

Tsumura H 等人：“针对鼠 FRP-1/CD98/4F2 重链的单克隆抗体的分离和表征”免疫学和细胞生物学。

Hideki Tsumura, Shoji Kimura, Mitsuo Kawano, Masato Tsurudome, Keishiro Shimura, Yasuhiko Ito: "Mouse alloantigen Ly10 is identical to murine fusion regulatory protein-1 (mFRP-1)/4F2/CD98 : Aberrant expression of mFRP-1/Ly10 allotypes in cells derived fro

Hideki Tsumura、Shoji Kimura、Mitsuo Kawano、Masato Tsurudome、Keishiro Shimura、Yasuhiko Ito：“小鼠同种异体抗原 Ly10 与小鼠融合调节蛋白 1 (mFRP-1)/4F2/CD98 相同：mFRP-1/Ly10 同种异型的异常表达

Hideki Tsumura, Mitsuo Kawano, Masanori Tajima, Takashi Kusaura, Yuuji Kozuka, Satoshi Yoshimura, Hiroshi Komada, Masato Tsurudome, Machiko Nishio, Shigeru Kusagawa, Keishirou Shimura, Yasuhiko Ito: "Isolation and characterization oh monoclonal antibodies

Hideki Tsumura、Mitsuo Kawano、Masanori Tajima、Takashi Kusaura、Yuuji Kozuka、Satoshi Yoshimura、Hiroshi Komada、Masato Tsurudome、Machiko Nishio、Shigeru Kusakawa、Keishiro Shimura、Yasuhiko Ito：“单克隆抗体的分离和表征