A study for the suppressive effect of the peptide growth factors on radiation-induced apoptosis.

我们发现肽类生长因子如VEGF抑制γ射线诱导的造血细胞凋亡。为了阐明VEGF抑制作用的分子机制，我们检测了bel家族的mRNA水平和蛋白水平，通过北方印迹和西方印迹杂交分析，发现BCL-2、BCL-XL、MCL-1的mRNA和蛋白水平在人白血病细胞系CMK 86细胞中均升高，而BCL-2或BCL-XL的mRNA和蛋白水平在人白血病细胞系CMK 86细胞中升高。MCL-1可能参与了VEGF抑制凋亡细胞死亡的作用.我们从CMK 86 cDNA文库中克隆了一个新的Kruppel型锌指基因ZK-7，该基因由VEGF诱导。ZK-7蛋白由298个氨基酸组成，N端含有KRAB（Kruppel associated box）-A盒，C端含有7个C_2H_2型锌指结构域。ZK-7基因在Popsis信号通路中的功能仍有待阐明。

We have found that peptide growth factors such as VEGF suppress the apoptotic cell death of the hematopoietic cells induced by exposure to gamma-rays.1. To elucidate the molecular mechanisns of the suppressive effects of VEGF,we examined the mRNA level and the protein level of the bel family (BCL-2, BCL-XL,MCL-1) by Northern blot and Western blot hybridization analysis.The mRNA level and the protein level of MCL-1 were increased in the CMK86 cells, a human leukemia cell line, stimulated by VEGF.Those of BCL-2 or BCL-XL,however, did not change by VEGF.MCL-1 may be involved in the suppressive effect of VEGF on apoptotic cell death.2. We cloned a novel Kruppel-type zinc finger gene ZK-7, induced by VEGF from a CMK86 cDNA library. The deduced ZK-7 protein has 298 amino acids, and has KRAB (Kruppel associated box) -A box at the N-terminus and seven C_2H_2-type zinc-finger domain at the C-terminus. The function of the ZK-7 gene on the signaling pathway of popsis remains to be elucidated.