Role of 1ymphocytes in airway inflammation and hyperresponsiveness induced by diesel exhaust particles.

1淋巴细胞在柴油机尾气颗粒诱导的气道炎症和高反应性中的作用。

• 批准号: 08680591
• 负责人: TAKANO Hirohisa
• 金额: $ 1.54万
• 依托单位: National Institute For Environmental Studies
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08680591/
• 关键词: dicsel exhieus particles; brenchial asthma; eosinophilic airway inflimmation; lymphocytos; cytokine; interleukin-5; surfoce markers; インターロイキン-5; ディーゼル排気微粒子(DEP); アレルギー性喘息; IL-5; CO4; IgG1

Previous experimental studies have suggested that nasal instillation of diesel exhaust particles (DEP) can enhance nasal lgE response and cytokine production ; However, there is no experimental evidence for the relation of DEP to allergic asthma. We investigated the effects of DEP inoculated intratracheally on antigen-induced airway inflammation, local expression of cytokine proteins, and antigen-specific immunoglobulin production in mice. DEP aggravated ovalbumin-induced airway inflammation characterized by infiltration of eosinophils and lymphocytes and an increase in goblet cells in bronchial epithelium. DEP with antigen markedly increased interleukin. 5 (lL-5) protein levels in lung tissue and bronchoalveolar lavage supernatants compared with either antigen or DEP alone. The combination of DEP and antigen induced significant increases in local expression of IL-4, granulocyte macrophage-colony stimulating factor (GM-CSF), and IL-2, whereas expression of interferon-gamma was not affected. In addition, DEP exhibited adjuvant activity for the antigen-specific production of lgG and lgE.These results provide the first experimental evidence that DEP can enhance the manifestations of allergic asthma. The enhancement may be mediated mainly by the increased local expression of IL-5, and also by the modulated expression of lL-4, GM-CSF,and lL-2.

以往的实验研究表明，鼻腔滴注柴油机尾气颗粒物（DEP）可增强鼻粘膜IgE反应和细胞因子的产生，但DEP与过敏性哮喘的关系尚缺乏实验证据。我们研究了气管内接种DEP对小鼠抗原诱导的气道炎症、细胞因子蛋白的局部表达和抗原特异性免疫球蛋白产生的影响。DEP可加重卵白蛋白诱导的气道炎症，其特征为嗜酸性粒细胞和淋巴细胞浸润以及支气管上皮杯状细胞增多。DEP与抗原显着增加白细胞介素。5（1 L-5）蛋白水平。DEP和抗原的组合诱导IL-4、粒细胞巨噬细胞集落刺激因子（GM-CSF）和IL-2的局部表达显著增加，而干扰素-γ的表达不受影响。此外，DEP对IgG和IgE的抗原特异性产生具有佐剂活性，这些结果为DEP可增强过敏性哮喘的表现提供了第一个实验证据。这种增强可能主要由IL-5的局部表达增加介导，也可能由IL-4、GM-CSF和IL-2的调节表达介导。

项目成果

高野 裕久: "ディーゼル排気微粒子によるアレルギーの増悪とその機序" 臨床免疫. 29. 1526-1532 (1997)

Hirohisa Takano：“柴油机尾气颗粒引起的过敏加剧及其机制”《临床免疫学》29。1526-1532（1997）。